Bioaccumulation and localization of exogenous cadmium in a teleost by electron microscopy (TEM) and its specific quantitation by electron probe X-ray microanalysis (EPMA)

A cadmium bioconcentration study was carried out in a fresh water teleost, Colisa fasciatus, to study the bioaccumulation kinetics and fate of exogenous cadmium (Cd) in biological tissues. Study shows that on exposure of the fish to a sublethal concentration of cadmium in test water, Cd uptake resul...

Full description

Saved in:
Bibliographic Details
Published in:Bioorganic & medicinal chemistry 2000-03, Vol.8 (3), p.475-482
Main Authors: TAYAL, A. K, KAUR, I, MATHUR, R. P
Format: Article
Language:English
Subjects:
Agnatha. Pisces
Animal, plant and microbial ecology
Animals
Applied ecology
Biological and medical sciences
Cadmium - pharmacokinetics
Cations, Divalent - metabolism
Cations, Monovalent - metabolism
Ecotoxicology, biological effects of pollution
Effects of pollution and side effects of pesticides on vertebrates
Electron Probe Microanalysis
Fishes - metabolism
Fundamental and applied biological sciences. Psychology
Gills - chemistry
Gills - cytology
Gills - ultrastructure
Liver - chemistry
Liver - cytology
Microscopy, Electron
Muscles - chemistry
Muscles - cytology
Silver Compounds
Silver Staining
Time Factors
Tissue Distribution
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A cadmium bioconcentration study was carried out in a fresh water teleost, Colisa fasciatus, to study the bioaccumulation kinetics and fate of exogenous cadmium (Cd) in biological tissues. Study shows that on exposure of the fish to a sublethal concentration of cadmium in test water, Cd uptake results in its bioconcentration in gills, liver and muscle tissues. To explore whether the accumulated Cd reaches the membranes or inside the cells, transmission electron microscopy (TEM) of the thin sections of tissues was done after histochemical localization of Cd in cells by modified SST method. TEM studies of sections of gills, liver and muscle tissues showed the deposits of exogenous Cd (visualized as dense clouds) in biological cells. This suggests the presence of free or loosely bound Cd on the membranes and inside the cells, which in the presence of Na2S is converted into insoluble metal sulfides. Electron probe X-ray microanalysis (EPMA) studies confirmed the presence of Cd on the membrane surface as well as inside the cells of bioindicator organs suggesting involvement of membrane transport of exogenous Cd inside the cells and its deposition as loosely bound insoluble metal complexes.
ISSN:0968-0896
1464-3391
DOI:10.1016/S0968-0896(99)00296-5