Isolation and characterization of Dorin M, a lectin from plasma of the soft tick Ornithodoros moubata

• Published in: Insect biochemistry and molecular biology 2000-03, Vol.30 (3), p.195-205
• Main Authors: Kovář, Vojtěch, Kopáček, Petr, Grubhoffer, Libor
• Format: Article
• Language: English
• Subjects: Animals; Argasidae; Hemagglutination Inhibition Tests; Hemagglutination Tests; Lectin; Lectins - chemistry; Lectins - immunology; Lectins - isolation & purification; Mice; Ornithodoros moubata; Sialic acid; Tick plasma; Ticks - chemistry
• ISSN: 0965-1748; 1879-0240
• DOI: 10.1016/S0965-1748(99)00107-1

A lectin with high hemagglutinating activity, which we have named Dorin M, was identified in the plasma of the soft tick Ornithodoros moubata. The activity of the plasma lectin could be efficiently inhibited by sialic acid, N-acetyl- d-hexosamines and sialoglycoproteins. Dorin M was purified to homogeneity using two different isolation systems: affinity chromatography on a column of bovine submaxillary mucin conjugated to Sepharose 4B with specific elution by N-acetyl- d-glucosamine and chromatography on Blue-Sepharose followed by anion exchange FPLC on a MonoQ column. The purified lectin is a glycoprotein which, in the native state, forms aggregates with molecular mass of about 640 kDa. Non-reducing SDS PAGE revealed that the lectin consists of two noncovalently bound subunits migrating closely around 37 kDa. Dorin M is a glycoprotein, probably modified by N-type glycosylation. After chemical deglycosylation, only one band of about 32 kDa was detected. Dorin M is the first lectin purified from ticks.