Insertion site preferences of the P transposable element in Drosophila melanogaster

We determined the genomic sequence at the site of insertion in 2,266 unselected P element insertion events. Estimating physical properties of the genomic DNA at these insertion sites—such as base composition, bendability, A-philicity, protein-induced deformability, and B-DNA twist—revealed that they...

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Published in:Proceedings of the National Academy of Sciences - PNAS 2000-03, Vol.97 (7), p.3347-3351
Main Authors: Liao, Guo-chun, Rehm, E. Jay, Rubin, Gerald M.
Format: Article
Language:English
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Summary:We determined the genomic sequence at the site of insertion in 2,266 unselected P element insertion events. Estimating physical properties of the genomic DNA at these insertion sites—such as base composition, bendability, A-philicity, protein-induced deformability, and B-DNA twist—revealed that they differ significantly from average chromosomal DNA. By examining potential hydrogen bonding sites in the major groove, we identified a 14-bp palindromic pattern centered on the 8-bp target site duplication that is generated by P element insertion. Our results suggest that the P-element transposition mechanism has a two-fold dyad symmetry and recognizes a structural feature at insertion sites, rather than a specific sequence motif.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.97.7.3347