An immunochemical assay to detect DNA damage in bovine sperm

An immunochemical assay has been developedto detect oxidative damage in bovine sperm DNA. Sperm DNA contains a large amount of oxidative damage as a result of exposure to exogenous agents, but damage also can caused by normal metabolic processes and the absence of DNA repair in the later stages of s...

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Bibliographic Details
Published in:Journal of andrology 2000-03, Vol.21 (2), p.250-257
Main Authors: van der Schans, G. P, Haring, R, van Dijk-Knijnenburg, H. C, Bruijnzeel, P. L, den Daas, N. H
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
bull fertility
Cattle
DNA Damage
DNA damage immuno‐assay
Enzyme-Linked Immunosorbent Assay
Fertility
Fundamental and applied biological sciences. Psychology
Immunohistochemistry
Insemination, Artificial
intrinsic sperm quality
Male
Mammalian male genital system
Morphology. Physiology
Oxidative Stress
Predictive assay
single‐strand DNA specific antibodies
Spermatozoa - metabolism
Vertebrates: reproduction
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Summary:An immunochemical assay has been developedto detect oxidative damage in bovine sperm DNA. Sperm DNA contains a large amount of oxidative damage as a result of exposure to exogenous agents, but damage also can caused by normal metabolic processes and the absence of DNA repair in the later stages of spermatogenesis. A freeze‐thaw procedure performed on extended bovine sperm in straws did not induce additional DNA damage immediately after thawing compared with nonfrozen extended sperm. The data suggest that the amount of oxidative damage correlated to the percentage of artificially inseminated cows returning to service within 56 days postinsemination, because a number of sires with high sperm concentrations had a large variation in fertility after artificial insemination. These observations have led to the conclusion that by measuring DNA damage in thawed sperm, one might predict the fertility of bulls with high semen concentration.
ISSN:0196-3635
1939-4640
DOI:10.1002/j.1939-4640.2000.tb02102.x