LC separation of calcipotriol from its photodegradation products and protection possibilities using adjuvants

Mobile phase optimization and reversed-phase column characteristics were used to separate photodegradation products from the parent compound, 24-cyclopropyl-9-,10-secochola-5,7,10(19),22-tetraene-1α,3β,24-triol (calcipotriol). Separation between calcipotriol and its degradation products was obtained...

Full description

Saved in:
Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2001-08, Vol.26 (1), p.31-41
Main Authors: Cirunay, Jesusa Joyce N, Vander Heyden, Yvan, Plaizier-Vercammen, Jacqueline
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
Calcipotriol
Calcitriol - analogs & derivatives
Calcitriol - analysis
Chromatography, High Pressure Liquid - methods
Dermatologic Agents - analysis
Drug Stability
General pharmacology
Light
Medical sciences
Pharmacology. Drug treatments
Photodegradation
Photostability
Sunscreen
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Mobile phase optimization and reversed-phase column characteristics were used to separate photodegradation products from the parent compound, 24-cyclopropyl-9-,10-secochola-5,7,10(19),22-tetraene-1α,3β,24-triol (calcipotriol). Separation between calcipotriol and its degradation products was obtained with an acetonitrile/water (53:47, v/v) mobile phase on a C 18 Hypersil ODS column (250 mm length, 4.6 mm id, 5 μm particle size) and a flow rate of 1 ml/min. Using this system, the influence of commonly used solvents in dermatology on degradation was studied. The addition of a UV filter in two concentrations was also evaluated for its possible protective effect to light exposure. Propylene glycol and polyethylene glycol 400 decreased the speed of degradation. The sunscreen 2-hydroxy-4-methoxybenzophenone affords a protection proportional to the filter concentration used in the study
ISSN:0731-7085
1873-264X
DOI:10.1016/S0731-7085(01)00390-9