Identification of fibroblast growth factor-5 as an overexpressed antigen in multiple human adenocarcinomas

Methodology for identifying tumor-associated antigens recognized by T cells has been successfully used to clone antigens from melanoma cells. Similar efforts for nonmelanoma tumors have had limited success with few antigens identified. To identify potentially relevant tumor-associated antigens expre...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 2001-07, Vol.61 (14), p.5511-5516
Main Authors: HANADA, Ken-Ichi, PERRY-LALLEY, Donna M, OHNMACHT, Galen A, BETTINOTTI, Maria P, YANG, James C
Format: Article
Language:English
Subjects:
Adenocarcinoma - immunology
Adenocarcinoma - pathology
Adult
Animals
Antibodies - immunology
Antineoplastic agents
Biological and medical sciences
Carcinoma, Renal Cell - immunology
Carcinoma, Renal Cell - pathology
Clone Cells
Cloning, Molecular
COS Cells
DNA, Complementary - genetics
Fibroblast Growth Factor 5
Fibroblast Growth Factors - genetics
Fibroblast Growth Factors - immunology
Humans
Immunotherapy
Medical sciences
Neoplasm Metastasis - immunology
Pharmacology. Drug treatments
T-Lymphocytes, Cytotoxic - immunology
Tumor Cells, Cultured
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Summary:Methodology for identifying tumor-associated antigens recognized by T cells has been successfully used to clone antigens from melanoma cells. Similar efforts for nonmelanoma tumors have had limited success with few antigens identified. To identify potentially relevant tumor-associated antigens expressed in renal cell carcinoma cell lines, a tumor-specific CTL clone was established from tumor-infiltrating lymphocytes from a regressing pulmonary lesion. This CTL recognized nonmutated fibroblast growth factor-5 (FGF-5). Quantitative real-time reverse transcription PCR revealed that FGF-5 was overexpressed in the majority of renal cell carcinomas, as well as in some prostate carcinoma and breast carcinoma lines. FGF-5 expression by quantitative real-time reverse transcription PCR in normal tissues was below the recognition threshold for this CTL. As a normal protein with significant overexpression by multiple adenocarcinomas and little normal tissue expression, FGF-5 represents an immunotherapy target with potential utility against a broad array of nonmelanoma cancers.
ISSN:0008-5472
1538-7445