The Generation of Superoxide Anion in Blood Platelets in Response to Different Forms of Proteus mirabilis Lipopolysaccharide: Effects of Staurosporin, Wortmannin, and Indomethacin

Lipopolysaccharide (LPS), the major component of the outer membrane of Gram-negative bacteria may activate blood platelets. The aim of our study was to evaluate the effects of different forms of Proteus mirabilis LPS and isolated lipid A and polysaccharide part on the production of superoxide radica...

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Bibliographic Details
Published in:Thrombosis research 2001-07, Vol.103 (2), p.149-155
Main Authors: Zielinski, Tomasz, Wachowicz, Barbara, Saluk-Juszczak, Joanna, Kaca, Wieslaw
Format: Article
Language:English
Subjects:
Androstadienes - pharmacology
Animals
Biological and medical sciences
Blood and lymphatic vessels
Blood Platelets - drug effects
Blood Platelets - metabolism
Cardiology. Vascular system
Cyclooxygenase Inhibitors - pharmacology
Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous
Enzyme Activation - drug effects
Enzyme Inhibitors - pharmacology
Indomethacin
Indomethacin - pharmacology
Lipopolysaccharide
Lipopolysaccharides - pharmacology
Medical sciences
Phosphatidylinositol 3-Kinases - antagonists & inhibitors
Platelets
Protein Kinase C - antagonists & inhibitors
Proteus mirabilis - chemistry
Signal Transduction - drug effects
Staurosporin
Staurosporine - pharmacology
Superoxides - metabolism
Swine
Wortmannin
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Summary:Lipopolysaccharide (LPS), the major component of the outer membrane of Gram-negative bacteria may activate blood platelets. The aim of our study was to evaluate the effects of different forms of Proteus mirabilis LPS and isolated lipid A and polysaccharide part on the production of superoxide radicals in blood platelets and to estimate the role staurosporin, wortmannin and indomethacin on this process. We compared the generation of superoxide radicals in platelets treated with LPS after preincubation with inhibitors of the signal transduction pathways, namely staurosporin (inhibitor of protein kinase C), wortmannin (inhibitor of phosphoinositide 3-kinase), and indomethacin (inhibitor of cycloxygenase). Our results demonstrate that all LPS molecules and their fragments caused a stimulation of O 2 − generation in platelets ( P
ISSN:0049-3848
1879-2472
DOI:10.1016/S0049-3848(01)00282-1