Nanopores and nucleic acids: prospects for ultrarapid sequencing

DNA and RNA molecules can be detected as they are driven through a nanopore by an applied electric field at rates ranging from several hundred microseconds to a few milliseconds per molecule. The nanopore can rapidly discriminate between pyrimidine and purine segments along a single-stranded nucleic...

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Bibliographic Details
Published in:Trends in biotechnology (Regular ed.) 2000-04, Vol.18 (4), p.147-151
Main Authors: Deamer, David W, Akeson, Mark
Format: Article
Language:English
Subjects:
Bacterial Toxins - chemistry
Biological and medical sciences
Biotechnology
Exobiology
Forecasting
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Hemolysin Proteins - chemistry
Methods. Procedures. Technologies
nanopore
Nanopores
Nucleic acid sequencing
nucleic acids
Nucleic Acids - analysis
Nucleic Acids - chemistry
Purines - chemistry
Pyrimidines - chemistry
RNA - analysis
Sequence Analysis - instrumentation
Sequence Analysis - methods
Sequence Analysis, DNA - instrumentation
Sequence Analysis, DNA - methods
Single molecule detection
Space life sciences
Synthetic digonucleotides and genes. Sequencing
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Summary:DNA and RNA molecules can be detected as they are driven through a nanopore by an applied electric field at rates ranging from several hundred microseconds to a few milliseconds per molecule. The nanopore can rapidly discriminate between pyrimidine and purine segments along a single-stranded nucleic acid molecule. Nanopore detection and characterization of single molecules represents a new method for directly reading information encoded in linear polymers. If single-nucleotide resolution can be achieved, it is possible that nucleic acid sequences can be determined at rates exceeding a thousand bases per second.
ISSN:0167-7799
1879-3096
DOI:10.1016/S0167-7799(00)01426-8