Localization of Enteroviral Antigen in Myocardium and Other Tissues from Patients with Heart Muscle Disease by an Improved Immunohistochemical Technique

SUMMARY The association of enterovirus infection and heart muscle diseases has been investigated extensively by detection of viral genomic RNA using nucleic acid hybridization and the reverse transcription-polymerase chain reaction. To further understand the role of enterovirus and its persistence i...

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Published in:The journal of histochemistry and cytochemistry 2000-05, Vol.48 (5), p.579-584
Main Authors: Zhang, Hongyi, Li, Yanwen, Peng, Tianqing, Aasa, Malen, Zhang, Li, Yang, Yingzhen, Archard, Leonard C
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - metabolism
Antibody Specificity
Antigens, Viral - analysis
Capsid - analysis
Capsid Proteins
Cardiomyopathy, Dilated - virology
Enterovirus - classification
Enterovirus - isolation & purification
Heart - virology
Histocytological Preparation Techniques
Humans
Immunohistochemistry - methods
Kidney - chemistry
Liver - chemistry
Lung - chemistry
Myocarditis - complications
Myocarditis - diagnosis
Myocardium - chemistry
Organ Specificity
Pancreas - chemistry
Reproducibility of Results
Serotyping
Spleen - chemistry
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Summary:SUMMARY The association of enterovirus infection and heart muscle diseases has been investigated extensively by detection of viral genomic RNA using nucleic acid hybridization and the reverse transcription-polymerase chain reaction. To further understand the role of enterovirus and its persistence in these diseases, an immunohistochemical technique was optimized to investigate the expression of viral capsid proteins in situ. A monoclonal antibody (5-D8/1) against an epitope in the N-terminus of capsid protein VP1, conserved in the enterovirus genus, was employed. To enhance sensitivity, the EnVison system was used to detect antigen-antibody complex. VP1 was detected in formalin-fixed, paraffin-embedded endomyocardial biopsy or postmortem myocardial tissues and in liver, spleen, lung, kidney, and pancreas from patients with myocarditis or dilated cardiomyopathy, but not from controls. VP1 was localized in cytoplasm of myofibers, often adjacent to necrosis and infiltrate in myocarditis, and was clustered or scattered in dilated cardiomyopathy. This technique can be used for a definitive laboratory diagnosis of enterovirus-associated diseases and for studying the mechanisms of virus persistence in chronic myocardial disease.
ISSN:0022-1554
1551-5044
DOI:10.1177/002215540004800501