Extension of Cell Life-Span and Telomere Length in Animals Cloned from Senescent Somatic Cells

The potential of cloning depends in part on whether the procedure can reverse cellular aging and restore somatic cells to a phenotypically youthful state. Here, we report the birth of six healthy cloned calves derived from populations of senescent donor somatic cells. Nuclear transfer extended the r...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2000-04, Vol.288 (5466), p.665-669
Main Authors: Lanza, Robert P., Cibelli, Jose B., Blackwell, Catherine, Cristofalo, Vincent J., Francis, Mary Kay, Baerlocher, Gabriela M., Mak, Jennifer, Schertzer, Michael, Chavez, Elizabeth A., Sawyer, Nancy, Lansdorp, Peter M., West, Michael D.
Format: Article
Language:English
Subjects:
Animals
B lymphocytes
Biological and medical sciences
Biotechnology
Blood cells
Blotting, Southern
Cattle - genetics
Cell Division
Cell nucleus
Cells
Cells, Cultured
Cellular Senescence
Clone Cells
Cloning
Cloning, Organism
DNA, Complementary
Embryo Transfer
EPC-1 gene
Eye Proteins
Female
Fetus
Fibroblasts
Flow Cytometry
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Hepatocytes
In Situ Hybridization, Fluorescence
Longevity
Matched-Pair Analysis
Methods. Procedures. Technologies
Miscellaneous
Nerve Growth Factors
Nuclear Transfer Techniques
Proteins - genetics
RNA, Messenger - genetics
RNA, Messenger - metabolism
Serpins - genetics
Somatic cells
Synthetic digonucleotides and genes. Sequencing
Telomerase
Telomere - ultrastructure
Telomeres
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Summary:The potential of cloning depends in part on whether the procedure can reverse cellular aging and restore somatic cells to a phenotypically youthful state. Here, we report the birth of six healthy cloned calves derived from populations of senescent donor somatic cells. Nuclear transfer extended the replicative life-span of senescent cells (zero to four population doublings remaining) to greater than 90 population doublings. Early population doubling level complementary DNA-1 (EPC-1, an age-dependent gene) expression in cells from the cloned animals was 3.5- to 5-fold higher than that in cells from age-matched (5 to 10 months old) controls. Southern blot and flow cytometric analyses indicated that the telomeres were also extended beyond those of newborn (
ISSN:0036-8075
1095-9203
DOI:10.1126/science.288.5466.665