Automated flow-injection spectrophotometric determination of catecholamines (epinephrine and isoproterenol) in pharmaceutical formulations based on ferrous complex formation

A novel automated flow-injection spectrophotometric method for the determination of catecholamines (epinephrine and isoproterenol) has been developed based on the formation of their coloured complexes with Fe(II) in aminoacetic-carbonate buffer pH 8.3 and measuring of the absorbance peaks at the λ m...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2000-06, Vol.22 (5), p.781-789
Main Authors: Solich, P., Polydorou, Ch.K., Koupparis, M.A., Efstathiou, C.E.
Format: Article
Language:English
Subjects:
Adrenaline
Analysis
Biological and medical sciences
Calibration
Content uniformity
Epinephrine
Epinephrine - analysis
Ferrous complex
Ferrous Compounds - chemistry
Flow injection
Flow Injection Analysis
General pharmacology
Isoprenaline
Isoproterenol
Isoproterenol - analysis
Medical sciences
Pharmaceutical analysis
Pharmaceutical Preparations - chemistry
Pharmacology. Drug treatments
Spectrophotometry
Spectrum Analysis - methods
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Summary:A novel automated flow-injection spectrophotometric method for the determination of catecholamines (epinephrine and isoproterenol) has been developed based on the formation of their coloured complexes with Fe(II) in aminoacetic-carbonate buffer pH 8.3 and measuring of the absorbance peaks at the λ max of 530 nm. A fully automated FIA system controlled by home-made software (FIA-MOD) was used for optimising the chemical and manifold parameters and running of routine measurements. The calibration graph was linear in the range of 5–200 mg l −1 for epinephrine with an RSD of 0.24% ( n=5; c=150 mg l −1) and 10–300 mg l −1 for isoproterenol with an RSD of 0.13% ( n=5; c=200 mg l −1). Measurement throughput was 120 h −1 ensuring a sample throughput of 40 h −1 analysed in triplicate. Common excipients for tablets and injections were found not interfering. The proposed method was applied for the assay of various commercial pharmaceutical formulations containing epinephrine and isoproterenol and for the content uniformity test for the isoproterenol tablets. The assay results with RSD 2–4% ( n=3) were comparable with those obtained with the official USP XXIII methods (mean difference 1.9%).
ISSN:0731-7085
1873-264X
DOI:10.1016/S0731-7085(00)00291-0