Acquisition of the monocyte/macrophage phenotype in human mesangial cells

The function of intrinsic glomerular cells in active glomerular inflammation may be similar to that of monocytes/macrophages. Mesangial cells have phagocytic properties and release numerous mediators. In this study we examined whether human mesangial cells (hMCs) express a monocyte/macrophage phenot...

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Bibliographic Details
Published in:The Journal of laboratory and clinical medicine 2001-09, Vol.138 (3), p.193-199
Main Authors: Watanabe, Susumu, Yoshimura, Ashio, Inui, Kiyoko, Yokota, Naoko, Liu, Yan, Sugenoya, Youichi, Morita, Hiroyuki, Ideura, Terukuni
Format: Article
Language:English
Subjects:
Acute Disease
Biological and medical sciences
Cell Count
Flow Cytometry
Fluorescent Antibody Technique, Indirect
Glomerular Mesangium - cytology
Glomerular Mesangium - drug effects
Glomerular Mesangium - metabolism
Glomerulonephritis
Glomerulonephritis, IGA - metabolism
Humans
Lupus Nephritis - metabolism
Macrophages - cytology
Male
Medical sciences
Middle Aged
Monocytes - cytology
Nephrology. Urinary tract diseases
Nephropathies. Renovascular diseases. Renal failure
Phenotype
Platelet-Derived Growth Factor - pharmacology
Proto-Oncogene Proteins c-sis
Receptor, Macrophage Colony-Stimulating Factor - genetics
Receptor, Macrophage Colony-Stimulating Factor - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
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Summary:The function of intrinsic glomerular cells in active glomerular inflammation may be similar to that of monocytes/macrophages. Mesangial cells have phagocytic properties and release numerous mediators. In this study we examined whether human mesangial cells (hMCs) express a monocyte/macrophage phenotype in active glomerular inflammation. We report that the proto-oncogene c-fms, the macrophage colony-stimulating factor (M-CSF) receptor, which is a characteristic gene of monocytes/macrophages, is expressed in hMCs. Normal unmanipulated hMCs express weak c-fms mRNA by reverse transcriptase–polymerase chain reaction (RT-PCR), and its expression increases after stimulation with platelet-derived growth factor-BB (PDGF-BB) and epidermal growth factor (EGF). The expression of c-fms was also demonstrated by flow cytometry with a specific polyclonal antibody. By immunohistochemistry, c-fms was prominently detected in acute glomerulonephritis, IgA nephritis, and lupus nephritis. These results indicate that hMCs express c-fms in active glomerular inflammation and are consistent with mesangial cells acquiring some macrophage-like characteristics in diseased states. (J Lab Clin Med 2001;138:193-9)
ISSN:0022-2143
1532-6543
DOI:10.1067/mlc.2001.116844