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Demonstration of the Nerve Distribution of the Masticatory Muscles in Rabbits (Oryctolagus cuniculus)

Two methods can be used in order to demonstrate the nerve distribution of an organ. One is the three‐dimensional reconstruction of the innervation pattern of the organ by tracing images of that organ from serial histological sections. The other is the in toto staining of the organ with subsequent cl...

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Bibliographic Details
Published in:Anatomia, histologia, embryologia histologia, embryologia, 2001-08, Vol.30 (4), p.225-229
Main Authors: Peker, T., Turgut, H. B., Gülekon, N., Anil, A.
Format: Article
Language:English
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Summary:Two methods can be used in order to demonstrate the nerve distribution of an organ. One is the three‐dimensional reconstruction of the innervation pattern of the organ by tracing images of that organ from serial histological sections. The other is the in toto staining of the organ with subsequent clearing of the muscles. In the present study, in order to visualize the nerve distribution of the organ, that organ was completely cleared and the nerve fibres were stained. Detailed morphological structure of the intramuscular nerve distribution of a certain region and its functions are of importance not only for anatomists and physiologists but also for clinicians. In this study eight New Zealand rabbits were used to visualize the intramuscular nerve distribution of the muscles involved in mastication (temporalis m., pterygoideus medialis m., digastricus m., retractor mandibulae m. and masseter m.). The main nerve bundle was observed entering into the muscle as a single trunk and dividing into three branches in the muscle. These branches were also observed dividing into several subbranches while going to the periphery. When the samples were examined under a stereomicroscope, ‘Y’‐, ‘I’‐ and ‘O’‐shaped communications between those branches were observed. Darstellung der Nervenverteilung in der Kaumuskulatur des Hasen (Oryctolagus cuniculus) Zwei Methoden sind bekannt, um das Innervationsmodell eines Organs darzustellen und zu analysieren. Die erste ist, eine mikroskopische Schnittserie von einem Organ herzustellen und mit Hilfe eines Computers den Verlauf eines Nerven zu rekonstruieren. Die zweite ist die Anfärbung von Nerven, um sie sichtbar zu machen. In dieser Studie wird die Färbemethode verwendet, um Nerven darzustellen. Für Anatomen, den Physiologen und den Kliniker ist es von Bedeutung, die anatomischen Lagebeziehungen intramuskulärer Nervenaufteilung zu wissen. Zur Untersuchung gelangen der Musculus temporalis, der Musculus pterygoideus medialis, der Musculus digastricus, der Musculus retractor mandibulae und der Musculus masseter von acht New Zealand‐Kaninchen. Jeder Muskel wird von einem einheitlichen Nervenast des Nervus madibularis versorgt, der in den Muskel eintritt und sich dann in zwei oder drei dünnere Äste aufteilt. Diese zweigen sich in der Peripherie in mehrere kleine Nervenästchen auf. Im Stereomikroskop ist es zu erkennen, dass die Nervenästchen ‘Y’‐, ‘I’‐ oder ‘O’‐förmige Verbindungen miteinander eingehen.
ISSN:0340-2096
1439-0264
DOI:10.1046/j.1439-0264.2001.00325.x