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In vitro correlate of immunity in a rabbit model of inhalational anthrax
A serological correlate of vaccine-induced immunity was identified in the rabbit model of inhalational anthrax. Animals were inoculated intramuscularly at 0 and 4 weeks with varying doses of Anthrax Vaccine Adsorbed (AVA) ranging from a human dose to a 1:256 dilution in phosphate-buffered saline (PB...
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| Published in: | Vaccine 2001-09, Vol.19 (32), p.4768-4773 |
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| Main Authors: | , , , , , , , , |
| Format: | Article |
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| cites | cdi_FETCH-LOGICAL-c474t-a7e53e96f685888931dc907e5f0a1b5d7bae55afaa05e8286d0e1071e2f2e60b3 |
| container_end_page | 4773 |
| container_issue | 32 |
| container_start_page | 4768 |
| container_title | Vaccine |
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| creator | Pitt, M.L.M Little, S.F Ivins, B.E Fellows, P Barth, J Hewetson, J Gibbs, P Dertzbaugh, M Friedlander, A.M |
| description | A serological correlate of vaccine-induced immunity was identified in the rabbit model of inhalational anthrax. Animals were inoculated intramuscularly at 0 and 4 weeks with varying doses of Anthrax Vaccine Adsorbed (AVA) ranging from a human dose to a 1:256 dilution in phosphate-buffered saline (PBS). At 6 and 10 weeks, both the quantitative anti-protective antigen (PA) IgG ELISA and the toxin-neutralizing antibody (TNA) assays were used to measure antibody levels to PA. Rabbits were aerosol-challenged at 10 weeks with a lethal dose (84–133 LD
50) of
Bacillus anthracis spores. All the rabbits that received the undiluted and 1:4 dilution of vaccine survived, whereas those receiving the higher dilutions of vaccine (1:16, 1:64 and 1:256) had deaths in their groups. Results showed that antibody levels to PA at both 6 and 10 weeks were significant (
P |
| doi_str_mv | 10.1016/S0264-410X(01)00234-1 |
| format | article |
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50) of
Bacillus anthracis spores. All the rabbits that received the undiluted and 1:4 dilution of vaccine survived, whereas those receiving the higher dilutions of vaccine (1:16, 1:64 and 1:256) had deaths in their groups. Results showed that antibody levels to PA at both 6 and 10 weeks were significant (
P<0.0001) predictors of survival.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/S0264-410X(01)00234-1</identifier><identifier>PMID: 11535328</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Administration, Inhalation ; Aerosols ; Animals ; Anthrax ; Anthrax - immunology ; Anthrax - prevention & control ; Anthrax - transmission ; Anthrax Vaccines - immunology ; Antibodies, Bacterial - biosynthesis ; Antibodies, Bacterial - immunology ; Antigens, Bacterial - immunology ; Bacillus anthracis ; Bacillus anthracis - immunology ; Bacterial Capsules - immunology ; Bacterial diseases ; Bacterial Toxins - immunology ; Biological and medical sciences ; Enzyme-Linked Immunosorbent Assay ; Experimental bacterial diseases and models ; Female ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; General aspects. Ontogeny. Phylogeny ; Immunobiology ; Immunoglobulin G - biosynthesis ; Immunoglobulin G - immunology ; Infectious diseases ; Injections, Intramuscular ; Male ; Mammals ; Medical sciences ; Models, Animal ; Neutralization Tests ; Rabbits ; Serological correlate ; Species Specificity ; Specific Pathogen-Free Organisms ; Spores, Bacterial ; Virulence</subject><ispartof>Vaccine, 2001-09, Vol.19 (32), p.4768-4773</ispartof><rights>2001</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-a7e53e96f685888931dc907e5f0a1b5d7bae55afaa05e8286d0e1071e2f2e60b3</citedby><cites>FETCH-LOGICAL-c474t-a7e53e96f685888931dc907e5f0a1b5d7bae55afaa05e8286d0e1071e2f2e60b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14153971$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11535328$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pitt, M.L.M</creatorcontrib><creatorcontrib>Little, S.F</creatorcontrib><creatorcontrib>Ivins, B.E</creatorcontrib><creatorcontrib>Fellows, P</creatorcontrib><creatorcontrib>Barth, J</creatorcontrib><creatorcontrib>Hewetson, J</creatorcontrib><creatorcontrib>Gibbs, P</creatorcontrib><creatorcontrib>Dertzbaugh, M</creatorcontrib><creatorcontrib>Friedlander, A.M</creatorcontrib><title>In vitro correlate of immunity in a rabbit model of inhalational anthrax</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>A serological correlate of vaccine-induced immunity was identified in the rabbit model of inhalational anthrax. Animals were inoculated intramuscularly at 0 and 4 weeks with varying doses of Anthrax Vaccine Adsorbed (AVA) ranging from a human dose to a 1:256 dilution in phosphate-buffered saline (PBS). At 6 and 10 weeks, both the quantitative anti-protective antigen (PA) IgG ELISA and the toxin-neutralizing antibody (TNA) assays were used to measure antibody levels to PA. Rabbits were aerosol-challenged at 10 weeks with a lethal dose (84–133 LD
50) of
Bacillus anthracis spores. All the rabbits that received the undiluted and 1:4 dilution of vaccine survived, whereas those receiving the higher dilutions of vaccine (1:16, 1:64 and 1:256) had deaths in their groups. Results showed that antibody levels to PA at both 6 and 10 weeks were significant (
P<0.0001) predictors of survival.</description><subject>Administration, Inhalation</subject><subject>Aerosols</subject><subject>Animals</subject><subject>Anthrax</subject><subject>Anthrax - immunology</subject><subject>Anthrax - prevention & control</subject><subject>Anthrax - transmission</subject><subject>Anthrax Vaccines - immunology</subject><subject>Antibodies, Bacterial - biosynthesis</subject><subject>Antibodies, Bacterial - immunology</subject><subject>Antigens, Bacterial - immunology</subject><subject>Bacillus anthracis</subject><subject>Bacillus anthracis - immunology</subject><subject>Bacterial Capsules - immunology</subject><subject>Bacterial diseases</subject><subject>Bacterial Toxins - immunology</subject><subject>Biological and medical sciences</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Experimental bacterial diseases and models</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>General aspects. Ontogeny. 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Psychology</topic><topic>Fundamental immunology</topic><topic>General aspects. Ontogeny. Phylogeny</topic><topic>Immunobiology</topic><topic>Immunoglobulin G - biosynthesis</topic><topic>Immunoglobulin G - immunology</topic><topic>Infectious diseases</topic><topic>Injections, Intramuscular</topic><topic>Male</topic><topic>Mammals</topic><topic>Medical sciences</topic><topic>Models, Animal</topic><topic>Neutralization Tests</topic><topic>Rabbits</topic><topic>Serological correlate</topic><topic>Species Specificity</topic><topic>Specific Pathogen-Free Organisms</topic><topic>Spores, Bacterial</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pitt, M.L.M</creatorcontrib><creatorcontrib>Little, S.F</creatorcontrib><creatorcontrib>Ivins, B.E</creatorcontrib><creatorcontrib>Fellows, P</creatorcontrib><creatorcontrib>Barth, J</creatorcontrib><creatorcontrib>Hewetson, J</creatorcontrib><creatorcontrib>Gibbs, P</creatorcontrib><creatorcontrib>Dertzbaugh, M</creatorcontrib><creatorcontrib>Friedlander, A.M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pitt, M.L.M</au><au>Little, S.F</au><au>Ivins, B.E</au><au>Fellows, P</au><au>Barth, J</au><au>Hewetson, J</au><au>Gibbs, P</au><au>Dertzbaugh, M</au><au>Friedlander, A.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro correlate of immunity in a rabbit model of inhalational anthrax</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2001-09-14</date><risdate>2001</risdate><volume>19</volume><issue>32</issue><spage>4768</spage><epage>4773</epage><pages>4768-4773</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>A serological correlate of vaccine-induced immunity was identified in the rabbit model of inhalational anthrax. Animals were inoculated intramuscularly at 0 and 4 weeks with varying doses of Anthrax Vaccine Adsorbed (AVA) ranging from a human dose to a 1:256 dilution in phosphate-buffered saline (PBS). At 6 and 10 weeks, both the quantitative anti-protective antigen (PA) IgG ELISA and the toxin-neutralizing antibody (TNA) assays were used to measure antibody levels to PA. Rabbits were aerosol-challenged at 10 weeks with a lethal dose (84–133 LD
50) of
Bacillus anthracis spores. All the rabbits that received the undiluted and 1:4 dilution of vaccine survived, whereas those receiving the higher dilutions of vaccine (1:16, 1:64 and 1:256) had deaths in their groups. Results showed that antibody levels to PA at both 6 and 10 weeks were significant (
P<0.0001) predictors of survival.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>11535328</pmid><doi>10.1016/S0264-410X(01)00234-1</doi><tpages>6</tpages></addata></record> |
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| ispartof | Vaccine, 2001-09, Vol.19 (32), p.4768-4773 |
| issn | 0264-410X 1873-2518 |
| language | eng |
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| source | Elsevier |
| subjects | Administration, Inhalation Aerosols Animals Anthrax Anthrax - immunology Anthrax - prevention & control Anthrax - transmission Anthrax Vaccines - immunology Antibodies, Bacterial - biosynthesis Antibodies, Bacterial - immunology Antigens, Bacterial - immunology Bacillus anthracis Bacillus anthracis - immunology Bacterial Capsules - immunology Bacterial diseases Bacterial Toxins - immunology Biological and medical sciences Enzyme-Linked Immunosorbent Assay Experimental bacterial diseases and models Female Fundamental and applied biological sciences. Psychology Fundamental immunology General aspects. Ontogeny. Phylogeny Immunobiology Immunoglobulin G - biosynthesis Immunoglobulin G - immunology Infectious diseases Injections, Intramuscular Male Mammals Medical sciences Models, Animal Neutralization Tests Rabbits Serological correlate Species Specificity Specific Pathogen-Free Organisms Spores, Bacterial Virulence |
| title | In vitro correlate of immunity in a rabbit model of inhalational anthrax |
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