Geographical patterns of genetic variation in two species of Stylosanthes Sw. using amplified fragment length polymorphism

Understanding the extent and distribution of genetic diversity within a species is essential for the development of effective conservation strategies. The objective of this study was to assess genetic variation using amplified fragment length polymorphisms (AFLP) in two species of the tropical legum...

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Bibliographic Details
Published in:Molecular ecology 2001-08, Vol.10 (8), p.1947-1958
Main Authors: Sawkins, M. C., Maass, B. L., Pengelly, B. C., Newbury, H. J., Ford-Lloyd, B. V., Maxted, N., Smith, R.
Format: Article
Language:English
Subjects:
AFLP
conservation
Evolution, Molecular
Fabaceae - classification
Fabaceae - genetics
Genetic Variation
geographical distribution
Geography
Phylogeny
Polymerase Chain Reaction - methods
Polymorphism, Genetic
Stylosanthes humilis
Stylosanthes viscosa
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Summary:Understanding the extent and distribution of genetic diversity within a species is essential for the development of effective conservation strategies. The objective of this study was to assess genetic variation using amplified fragment length polymorphisms (AFLP) in two species of the tropical legume genus Stylosanthes Sw. Annual, S. humilis (2n = 20) and perennial, S. viscosa (2n = 20) are found throughout tropical America, and are sympatric for much of their range of distribution. One hundred and eleven accessions, covering a wide geographical range, were selected for AFLP analysis. Binary data matrices derived from DNA banding patterns were analysed using the software programs ntsys‐pc and arlequin. Several accessions were found to be misidentified. Of the S. humilis accessions, the overall average similarity value was (0.72) slightly higher than the value obtained for S. viscosa (0.67). Cluster analysis and principal coordinate analysis grouped accessions from both species by geographical origin, with a few exceptions. Analysis of molecular variance (amova) in S. humilis revealed 59.4% of the variation among groups formed from the cluster analysis. This was highly significant (P 
ISSN:0962-1083
1365-294X
DOI:10.1046/j.0962-1083.2001.01347.x