Pulmonary Blast Injury Increases Nitric Oxide Production, Disturbs Arginine Metabolism, and Alters the Plasma Free Amino Acid Pool in Rabbits during the Early Posttraumatic Period

• Published in: Nitric oxide 2000-04, Vol.4 (2), p.123-128
• Main Authors: Žunić, Gordana, Pavlović, Radmila, Maličević, Živorad, Savić, Veljko, Cernak, Ibolja
• Format: Article
• Language: English
• Subjects: Amino Acids - blood; Animals; arginine; Arginine - metabolism; Blast Injuries - blood; Blast Injuries - metabolism; blast injury; Disease Models, Animal; Male; nitrates; Nitrates - blood; Nitric Oxide - metabolism; Nitrites - blood; Rabbits; Respiratory Distress Syndrome, Adult - blood; Respiratory Distress Syndrome, Adult - metabolism
• ISSN: 1089-8603; 1089-8611
• DOI: 10.1006/niox.2000.0276

Plasma nitrate + nitrite (nitrates), as final NO products, and free amino acid pool (FAAP) characteristics, as indicators of protein/amino acid metabolism, were analyzed in the early (30 min) period following blast injury. The experiments were performed on 27 rabbits subjected to pulmonary blast injury (experimental group) or not exposed to overpressure (controls). We report that pulmonary blast injury (PBI) induces prompt NO overproduction within a very early period. Increased arginine utilization via NO synthase, presumably associated with its cleavage by arginase, leads to the depletion of the arginine level in arterial plasma 30 min following PBI. Impaired balance between arginine utilization and release/resynthesis from endogenous sources causes disturbed nutritional status and urea cycle activity. Early identification and appropriate management of the changes in amino acid metabolism should be included in the evaluation of patients with blast injury. Furthermore, the results suggest that depleted arterial levels of arginine and NO overproduction may be helpful in diagnosis and prognosis of blast injury.