Cytoplasmic accumulation of flavonoids in flower petals and its relevance to yellow flower colouration

• Published in: Phytochemistry (Oxford) 2001-10, Vol.58 (3), p.403-413
• Main Authors: Markham, Kenneth R., Gould, Kevin S., Ryan, Ken G.
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Chromatography, High Pressure Liquid; Color; Compartmentation; Cytoplasm; Cytoplasm - metabolism; Eustoma grandiflorum; Flavonoid-protein binding; Flavonoids; Flavonoids - metabolism; Fundamental and applied biological sciences. Psychology; Lathyrus chrysanthus; Lisianthus; Magnoliopsida - metabolism; Metabolism; Metabolism. Physicochemical requirements; Microscopy, Fluorescence; Petals; Plant physiology and development; Spectrophotometry, Ultraviolet; Yellow colouration
• ISSN: 0031-9422; 1873-3700
• DOI: 10.1016/S0031-9422(01)00276-X

It is widely accepted that the mix of flavonoids in the cell vacuole is the source of flavonoid based petal colour, and that analysis of the petal extract reveals the nature and relative levels of vacuolar flavonoid pigments. However, it has recently been established with lisianthus flowers that some petal flavonoids can be excluded from the vacuolar mix through deposition in the cell wall or through complexation with proteins inside the vacuole, and that these flavonoids are not readily extractable. The present work demonstrates that flavonoids can also be compartmented within the cell cytoplasm. Using adaxial epidermal peels from the petals of lisianthus ( Eustoma grandiflorum), Lathyrus chrysanthus and Dianthus caryophyllus, light and laser scanning confocal microscopy studies revealed a significant concentration of petal flavonoids in the cell cytoplasm of some tissues. With lisianthus, flavonoid analyses of isolated protoplasts and vacuoles were used to establish that ca 14% of petal flavonoids are located in the cytoplasm (cf. 30% in the cell wall and 56% in the vacuole). The cytoplasmic flavonoids are predominantly acylated glycosides (cf. non-acylated in the cell wall). Flavonoid aggregation on a cytoplasmic protein substrate provides a rational mechanism to account for how colourless flavonoid glycosides can produce yellow colouration in petals, and perhaps also in other plant parts. High vacuolar concentrations of such flavonoids are shown to be insufficient. Flavonol glycosides have been found in the cell cytoplasm of lisianthus ( Eustoma grandiflorum) and Lathyrus chrysanthus flower petals. Interactions within the cytoplasm are proposed to account for yellow petal colouration resulting from “colourless” flavonols.