RAPD of controlled crosses and clones from the field suggests that hybrids are rare in the Salix alba–Salix fragilis complex

The polyploid Salix alba–Salix fragilis hybrid complex is rather difficult to study when using only morphological characters. Most of the features have a low diagnostic value for unambiguously identifying the hybrids, introgression patterns and population structures, though morphological traits have...

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Bibliographic Details
Published in:Heredity 2000-05, Vol.84 (5), p.555-563
Main Authors: Triest, Ludwig, de Greef, Bart, de Bondt, Ruth, van Slycken, Jos
Format: Article
Language:English
Subjects:
Belgium
Biomedical and Life Sciences
Biomedicine
Body Constitution
Chimera - genetics
Clone Cells
Crosses, Genetic
Cytogenetics
DNA - analysis
DNA, Plant - genetics
Ecology
Evolutionary Biology
genetic diversity
Genetic Variation
Human Genetics
hybridization
Hybridization, Genetic
Hybrids
introgression
Ordination
original-article
Plant Genetics and Genomics
Plants - genetics
Polymerase Chain Reaction
polymorphism
Polymorphism, Genetic
Polyploidy
Random Amplified Polymorphic DNA Technique
RAPD
Salix alba
Salix fragilis
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Summary:The polyploid Salix alba–Salix fragilis hybrid complex is rather difficult to study when using only morphological characters. Most of the features have a low diagnostic value for unambiguously identifying the hybrids, introgression patterns and population structures, though morphological traits have proved to be useful in making a hybrid index. Morphology and molecular variation from RAPDs were investigated in several case studies on willows from Belgium. A thorough screening of full-sib progenies of interspecific controlled crosses was made to select homologous amplification products. The selected amplified products proved to be useful in a principal coordinate analysis for the estimation of variability of hybrid progenies. On the basis of genetic similarities and ordination analysis, a method for the identification of clones in the field was established using presumed pure species and presumed introgressants. The chosen reference clones were checked against additional European samples of putative pure species to ensure the reliability of the method beyond a regional scale. The RAPDs suggested that both species have kept their gene pools well separated and that hybridization actually does not seem to be a dominating process. The observation that molecular markers do not always follow the morphological traits or allozyme data is discussed.
ISSN:0018-067X
1365-2540
0018-067X
DOI:10.1046/j.1365-2540.2000.00712.x