Localization of radiation-induced chromosomal breakpoints along human chromosome 1 using a combination of G-banding and FISH

Purpose : To determine the exact location of radiation-induced chromosomal breakpoints along the euchromatic or heterochromatic regions: G-light and G-dark bands, respectively. Materials and methods : The distribution of radiation-induced chromosomal breakpoints was scored in human lymphocytes irrad...

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Bibliographic Details
Published in:International journal of radiation biology 2000, Vol.76 (5), p.667-672
Main Author: Kiuru, C. Lindholm, A. Auvinen, S. Salomaa, A.
Format: Article
Language:English
Subjects:
Chromosome Aberrations
Chromosome Banding
Chromosome Breakage
Chromosomes - genetics
Chromosomes - radiation effects
Chromosomes, Human, Pair 1 - genetics
Chromosomes, Human, Pair 1 - radiation effects
DNA Damage - radiation effects
Gamma Rays
Humans
In Situ Hybridization, Fluorescence
Lymphocytes - radiation effects
Metaphase - radiation effects
Space life sciences
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Summary:Purpose : To determine the exact location of radiation-induced chromosomal breakpoints along the euchromatic or heterochromatic regions: G-light and G-dark bands, respectively. Materials and methods : The distribution of radiation-induced chromosomal breakpoints was scored in human lymphocytes irradiated in vitro with 3 Gy of gamma-radiation. Image analysis was applied to combine G-banded and FISH-painted images of the human chromosome 1. Results : A total of 195 chromosomal breakpoints in 176 cells with structural chromosomal aberrations was used for the present analysis. Radiation-induced breakpoints were found to be distributed randomly with respect to the p or q arms of chromosome 1 and specific band or band length, but more breakpoints were mapped to G-light than to G-dark bands, the difference being statistically significant. Conclusions : The results can well be interpreted in terms of concepts of existing models of nuclear architecture, chromatin structure and transcriptional activities of the chromatin, which can influence the induction of primary chromosomal aberrations by gamma-rays. Differential repair of randomly produced primary aberrations may also explain the non-random distribution of radiation-induced breakpoints.
ISSN:0955-3002
1362-3095
DOI:10.1080/095530000138330