Partial characterization of a non-proteinaceous, low molecular weight antigen of Eimeria tenella

A low molecular weight (LMW) antigen of Eimeria tenella, initially identified using a murine monoclonal antibody (mAb C(3)4F(1)) raised against E. tenella sporozoites, was partially characterized using enzymatic degradation. solvent extraction, and immunization into various inbred lines of mice. The...

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Bibliographic Details
Published in:Parasitology research (1987) 2000-06, Vol.86 (6), p.461-466
Main Authors: BARTA, J. R, TENNYSON, S. A, SCHITO, M. L, DANFORTH, H. D, MARTIN, D. S
Format: Article
Language:English
Subjects:
Animals
Antibodies, Protozoan
Antigens, Protozoan - chemistry
Biochemistry. Physiology. Immunology. Molecular biology
Biological and medical sciences
Eimeria tenella - immunology
Experimental protozoal diseases and models
Fundamental and applied biological sciences. Psychology
Genetic Linkage
Glycolipids - immunology
Immunologic Deficiency Syndromes
Infectious diseases
Male
Medical sciences
Mice
Mice, Inbred BALB C
Mice, Inbred CBA
Molecular Weight
Parasitic diseases
Protozoa
Protozoal diseases
X Chromosome
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Summary:A low molecular weight (LMW) antigen of Eimeria tenella, initially identified using a murine monoclonal antibody (mAb C(3)4F(1)) raised against E. tenella sporozoites, was partially characterized using enzymatic degradation. solvent extraction, and immunization into various inbred lines of mice. The LMW antigen could be isolated using Folch extraction (methanol/chloroform/ water) and the epitope recognized by mAb C(3)4F(1) was resistant to degradation by alpha-amylase, pronase, and proteinase K, but was sensitive to sodium m-periodate treatment or digestion using mixed glycosidases (from Turbo cornutus). These observations suggest that the antigenic epitope recognized by mAb C(3)4F(1) is carbohydrate-dependent and, based on our ability to isolate the LMW antigen by Folch extraction, the epitope probably resides on a polar glycolipid. The inability of sporozoite-immunized nude mice to elicit a serum antibody response to this molecule indicates that it acts as a T-dependent antigen. Furthermore, sporozoite-immunized male CBA/N mice (with an X-linked immunodeficiency) also failed to elicit a serum antibody response to this molecule, which is consistent with a carbohydrate antigenic epitope. We propose that this antigenic molecule be designated ET-GL1 to reflect its origin and probable structure (E. tenella glycolipid 1).
ISSN:0932-0113
1432-1955
DOI:10.1007/s004360050694