Controlling human polymorphonuclear leukocytes motility using microfabrication technology

We describe a new approach for controlling cell motility on a material surface. Transparent, photosensitive polyimide materials were used to fabricate physical structures on glass; cell motility was then followed over time using optical microscopy. Arrays of pillars and holes with 2 micron square, 4...

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Bibliographic Details
Published in:Journal of biomedical materials research 2000-09, Vol.51 (4), p.694-702
Main Authors: Tan, Jian, Shen, Hong, Carter, Katrina L., Saltzman, W. Mark
Format: Article
Language:English
Subjects:
Adhesion
Biocompatible Materials
Biological and medical sciences
Biotechnology
Cell culture
Cell Movement
Glass
Humans
In Vitro Techniques
Materials Testing
Medical sciences
microfabrication
Microscopy, Electron, Scanning
motility
neutrophil
Neutrophils - physiology
Neutrophils - ultrastructure
Optical microscopy
Photolithography
Photosensitivity
physical pattern
Plastic films
Polyimides
Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)
Surface Properties
Surface roughness
Technology. Biomaterials. Equipments. Material. Instrumentation
Transparency
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Summary:We describe a new approach for controlling cell motility on a material surface. Transparent, photosensitive polyimide materials were used to fabricate physical structures on glass; cell motility was then followed over time using optical microscopy. Arrays of pillars and holes with 2 micron square, 4‐μm height (or depth) separated by 10 μm were successfully patterned using photolithography. Neutrophils attached and spread on the smooth glass surface and surfaces with pillars. In contrast, cells were rounded and did not adhere to either smooth polyimide film or films with holes. The migration of neutrophils was much faster on holes than on polyimide surface, but it was significantly slower on pillars than on glass. These results suggest that physical patterning may be an effective tool to manipulate cell migration in the design of biomaterials for tissue engineering. © 2000 John Wiley & Sons, Inc. J Biomed Mater Res, 51, 694–702, 2000.
ISSN:0021-9304
1097-4636
DOI:10.1002/1097-4636(20000915)51:4<694::AID-JBM18>3.0.CO;2-N