MLL amplification in myeloid leukemias: A study of 14 cases with multiple copies of 11q23

We here report the clinical, cytogenetic, fluorescence in situ hybridization (FISH), and Southern blot data on 14 patients with a myeloid malignancy and structural aberration of chromosome band 11q23 associated with overrepresentation or amplification of the MLL gene. The number of copies of MLL var...

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Bibliographic Details
Published in:Genes chromosomes & cancer 2000-09, Vol.29 (1), p.40-47
Main Authors: Michaux, L, Wlodarska, I, Stul, M, Dierlamm, J, Mugneret, F, Herens, C, Beverloo, B, Verhest, A, Verellen-Dumoulin, C, Verhoef, G, Selleslag, D, Madoe, V, Lecomte, M, Deprijck, B, Ferrant, A, Delannoy, A, Marichal, S, Duhem, C, Dicato, M, Hagemeijer, A
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Blotting, Southern
Chromosomes, Human, Pair 11 - genetics
DNA-Binding Proteins - genetics
Female
Gene Amplification - genetics
Gene Dosage
Histone-Lysine N-Methyltransferase
Humans
Immunophenotyping
In Situ Hybridization, Fluorescence
Karyotyping
Leukemia, Myeloid - diagnosis
Leukemia, Myeloid - genetics
Leukemia, Myeloid - pathology
Male
Middle Aged
Myeloid-Lymphoid Leukemia Protein
Proto-Oncogenes
Retrospective Studies
Transcription Factors
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Description
Summary:We here report the clinical, cytogenetic, fluorescence in situ hybridization (FISH), and Southern blot data on 14 patients with a myeloid malignancy and structural aberration of chromosome band 11q23 associated with overrepresentation or amplification of the MLL gene. The number of copies of MLL varied from three (two cases) to a cluster consisting of multiple hybridization spots. Together with previous reports, available data indicate that amplification of 11q23/MLL is a recurrent genetic change in myeloid malignancy. It affects mainly elderly patients and is often associated with dysplastic bone marrow changes or with complex karyotypic aberrations, suggestive of genotoxic exposure. It is associated with a poor prognosis. In addition, FISH analysis of nine cases with additional 11q probes showed that the overrepresented chromosomal region is generally not restricted to MLL, and Southern blot analysis indicated that amplification does not involve a rearranged copy of this gene. The significance of MLL amplification and the mechanisms by which it could play a role in leukemogenesis and/or disease progression remain to be elucidated.
ISSN:1045-2257
1098-2264
DOI:10.1002/1098-2264(2000)9999:9999<::AID-GCC1003>3.3.CO;2-L