A linkage map of distal mouse chromosome 4 in the vicinity of Ssm1, a strain-specific modifier of methylation

Inactive chromosomal regions are often characterized by the presence of 5-methyl cytosine in CpG dinucleotides and by hypoacetylated histones. A direct biochemical link between CpG methylation and chromatin structure has now been established: MeCP2, a methyl cytosine-binding protein, can recruit his...

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Bibliographic Details
Published in:Mammalian genome 2000-08, Vol.11 (8), p.694-695
Main Authors: Engler, P, Storb, U
Format: Article
Language:English
Subjects:
5-methyl cytosine
Animals
Blotting, Southern
chromosome 4
Chromosome Mapping
Chromosomes - genetics
Crosses, Genetic
DNA - genetics
DNA - metabolism
DNA Methylation
Female
Genetic Linkage
histone deacetylase
histones
HRD342 gene
Male
MeCP2 protein
methyl cytosine-binding protein
Mice
Mice, Inbred C57BL
Mice, Inbred DBA
Mice, Transgenic
Microsatellite Repeats
Ssm1 gene
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Summary:Inactive chromosomal regions are often characterized by the presence of 5-methyl cytosine in CpG dinucleotides and by hypoacetylated histones. A direct biochemical link between CpG methylation and chromatin structure has now been established: MeCP2, a methyl cytosine-binding protein, can recruit histone deacetylases to methylated DNA. Little is known, however, about how methylation patterns are initially established. Understanding how the Ssm1 locus acts may provide a clue to how these patterns are established. Ssm1, for strain-specific modifier, is a locus that affects the methylation of certain transgene loci. When the HRD342 transgene is carried in certain inbred mouse strains such as C57BL/ 6J (hereafter referred to as B6), it is heavily methylated. Upon crossing to other strains such as DBA/2J (hereafter referred to as D2), the transgene loci become hypomethylated. By using crosses with recombinant inbred mice, the locus responsible for transgene methylation has been mapped to distal Chromosome (Chr) 4. We now report further localization of Ssm1 in preparation for positional cloning of this gene.
ISSN:0938-8990
1432-1777
DOI:10.1007/s003350010124