Distribution of ion transport mRNAs throughout murine nose and lung

Cystic Fibrosis/Pulmonary Research and Treatment Center, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 Evidence of absorptive or secretory ion transport in different respiratory regions of the mouse was sought by assessing the regional distributio...

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Bibliographic Details
Published in:American journal of physiology. Lung cellular and molecular physiology 2000-07, Vol.279 (1), p.14-L24
Main Authors: Rochelle, Lori G, Li, Dong Chen, Ye, Helen, Lee, Eddie, Talbot, Colleen R, Boucher, Richard C
Format: Article
Language:English
Subjects:
Animals
Bronchi - metabolism
Carrier Proteins - genetics
Cystic Fibrosis Transmembrane Conductance Regulator - genetics
Epithelial Sodium Channels
Esophagus - metabolism
Lung - metabolism
Mice
Mice, Inbred DBA
Mice, Inbred Strains
Nasal Cavity - metabolism
Pulmonary Alveoli - metabolism
RNA, Messenger - metabolism
Sodium Channels - genetics
Sodium-Potassium-Chloride Symporters
Thyroid Gland - metabolism
Tissue Distribution
Trachea - metabolism
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Summary:Cystic Fibrosis/Pulmonary Research and Treatment Center, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 Evidence of absorptive or secretory ion transport in different respiratory regions of the mouse was sought by assessing the regional distribution of -, -, and -epithelial sodium channel (ENaC; Na + absorptive), cystic fibrosis transmembrane conductor regulator (CFTR), and Na + -K + -2Cl cotransporter mRNAs. High levels of ENaC subunit expression were found in nasal surface epithelium and gland ducts. CFTR was expressed in both superficial nasal respiratory epithelium and glands. These results are consistent with basal amiloride-sensitive Na + absorption and cAMP-dependent Cl secretion in murine nasal epithelia. Expression of all three ENaC subunits increased progressively from trachea to terminal bronchioles. Intermediate levels of CFTR and cotransporter expression in bronchial epithelium diminished in bronchioles. The low abundance of CFTR mRNA throughout murine pulmonary epithelium is consistent with functional data that attributes Cl secretion predominantly to an alternative Cl channel. -ENaC as the only mRNA found in all regions of airway epithelia is consistent with the -subunit as requisite for Na + absorption, and the increased expression of -, -, and -ENaC in distal airways suggests a greater absorptive capability in this region. cystic fibrosis transmembrane conductor regulator; epithelial sodium channel; sodium potassium-chloride cotransporter; nasal
ISSN:1040-0605
1522-1504
DOI:10.1152/ajplung.2000.279.1.l14