Sperm motility index: a quick screening parameter from sperm quality analyser-IIB to rule out oligo- and asthenozoospermia in male fertility study

• Published in: Human reproduction (Oxford) 2000-08, Vol.15 (8), p.1727-1733
• Main Authors: Martínez, C., Mar, C., Azcárate, M., Pascual, P., Aritzeta, J.M., López-Urrutia, A.
• Format: Article
• Language: English
• Subjects: automated semen analysis; Automation; Biological and medical sciences; Evaluation Studies as Topic; Genital system. Mammary gland; Humans; Infertility, Male - diagnosis; Investigative techniques, diagnostic techniques (general aspects); Linear Models; Male; Medical sciences; Oligospermia - diagnosis; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Reproducibility of Results; Reproductive Techniques - instrumentation; semen quality; Sensitivity and Specificity; Sperm Motility; sperm motility index; sperm quality analyser; Spermatozoa - physiology
• ISSN: 0268-1161; 1460-2350; 1460-2350
• DOI: 10.1093/humrep/15.8.1727

The aim of this study was to evaluate the sperm quality analyser (SQA)-IIB, a new automated sperm analyser, and to compare its results with those obtained with a method based on the World Health Organization recommendations. Eighty-nine unprocessed semen samples and 53 selected sperm suspensions were analysed. Concentration, motility and morphology were evaluated using the routine laboratory method. The SQA-IIB measured the sperm motility index (SMI) and estimated the previously mentioned parameters. In the imprecision assay a maximal coefficient of variation (CV) of 18.8% was found. A semen sample with immunological factor showed a CV of 75.75%, which invalidates its use for these types of samples. A good correlation was obtained between SMI and concentration of progressively motile spermatozoa (CPMS) (r = 0.87), and a fair correlation with the other parameters. There was no statistically significant correlation between both methods for normal sperm morphology. The sensitivity and specificity of the SMI test in relation to CPMS were 96 and 84% respectively, for an SMI threshold value of 160. The results obtained make the SQA-IIB a good screening test to rule out oligozoospermia and asthenozoospermia when studying the male factor in the sterility outpatient clinics. However, the results suggested that it is not a valid method to evaluate morphology.