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Flavopiridol Potently Induces Small Cell Lung Cancer Apoptosis during S Phase in a Manner That Involves Early Mitochondrial Dysfunction
Purpose: Accumulating evidence indicates that small cell lung cancer (SCLC) is defective in many of the regulatory mechanisms that control cell cycle progression. The purpose of this study was to determine the effects of flavopiridol, a pan-cyclin-dependent kinase inhibitor, on growth and apoptosis...
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Published in: | Clinical cancer research 2003-10, Vol.9 (12), p.4586-4594 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Online Access: | Get full text |
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Summary: | Purpose: Accumulating evidence indicates that small cell lung cancer (SCLC) is defective in many of the regulatory mechanisms that
control cell cycle progression. The purpose of this study was to determine the effects of flavopiridol, a pan-cyclin-dependent
kinase inhibitor, on growth and apoptosis of SCLC cell lines.
Experimental Design: Cell growth was monitored using 3-(4,5dimethylthiazol-2yl)-2,5-diphenyl-tetrazolium bromide (MTT) and clonogenic assays.
Induction of apoptosis was assessed using multiple assays, including flow cytometric determination of DNA content and mitochondrial
membrane potential, terminal deoxynucleotide transferase-mediated dUTP nick end labeling (TUNEL), and Western blot analysis
of procaspase 3 and poly(ADP-ribose) polymerase cleavage.
Results: Flavopiridol induced growth inhibition and cytotoxicity in multiple SCLC cell lines, with an IC 50 of 50–100 n m and an LD 50 of 150–200 n m in 72-h MTT assays. The cytotoxicity seen in the MTT assay proved to be apoptosis by several criteria. Interestingly, inhibition
of caspase activation with the caspase inhibitor Boc-Asp(OMe)-CH 2 F reduced TUNEL labeling by 40% but did not have any effect on the loss of mitochondrial membrane potential (detected as early
as 4 h after drug exposure) or cytotoxicity in MTT assays. These results suggest that the primary event in flavopiridol-induced
apoptosis involves induction of mitochondrial dysfunction. Cells synchronized with aphidicolin at the G 1 -S border and treated with flavopiridol during S phase showed a marked increase in apoptosis compared with an asynchronous
population or a population treated during G 2 -M. Despite the increased apoptosis, a significant proportion of synchronized cells proceeded through S, G 2 -M, and into G 1 phase in the presence of flavopiridol, demonstrating that a high-grade cell cycle arrest is not required for apoptosis. Cells
synchronized at the G 1 -S border treated with a short exposure to flavopiridol also showed more than a 10-fold decrease in clonogenicity compared
with asynchronous cells treated identically.
Conclusions: Taken together, these data demonstrate that flavopiridol potently and selectively induces SCLC apoptosis preferentially during
S phase, in a manner that involves early mitochondrial dysfunction without a requirement for a high-grade block to cell cycle
progression. Furthermore, clonogenicity data suggests that prior S phase synchronization could be a highly effective way of
enhancing the |
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ISSN: | 1078-0432 1557-3265 |