Amphibian and mammal somatic-cell cloning: different species, common results?

Since the production of Dolly the sheep cloning methods for somatic cells have been thoroughly described and are becoming routine. However, the rate at which live clones are produced remains low in all mammalian species tested so far. Remarkably, irrespective of the cloning protocol or the donor-cel...

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Bibliographic Details
Published in:Trends in biotechnology (Regular ed.) 2003-11, Vol.21 (11), p.471-473
Main Authors: Loi, Pasqualino, Fulka, Josef, Ptak, Grazyna
Format: Article
Language:English
Subjects:
Acquisitions & mergers
Amphibia
Amphibians
Animals
Biological and medical sciences
Biotechnology
Breeding of animals
Chromatin - physiology
Cloning
Cloning, Organism
Cytoplasm - physiology
Embryo, Mammalian - physiology
Embryo, Nonmammalian
Epigenesis, Genetic
Epigenetics
Experiments
Female
Fundamental and applied biological sciences. Psychology
Gene expression
Genetic Techniques
Genomes
Male
Mammalia
Mammals
Mammals - genetics
Nuclear Transfer Techniques
Oocytes - physiology
Placenta
Pregnancy
Stem cells
Studies
Xenopus - genetics
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Summary:Since the production of Dolly the sheep cloning methods for somatic cells have been thoroughly described and are becoming routine. However, the rate at which live clones are produced remains low in all mammalian species tested so far. Remarkably, irrespective of the cloning protocol or the donor-cell type, all clones display common abnormalities, particularly in the placenta. The process is also complicated by early mortality of somatic-cell clones and the founder mammalian clone, Dolly the sheep, died in February 2003 aged six years. Based on published data and on our own experience, our view is that mammalian somatic-cell cloning and the pioneer nuclear-transfer data from amphibians have much in common. We suggest that the only way to improve nuclear reprogramming is to modify the chromatin structure of somatic cells before nuclear transfer, to provide the oocyte with a chromosomal structure that is more compatible with the natural reprogramming machinery of the oocyte.
ISSN:0167-7799
1879-3096
DOI:10.1016/j.tibtech.2003.09.007