Pituitary adenylate cyclase‐activating polypeptide inhibits cutaneous immune function

Epidermal nerves are closely associated with Langerhans cells (LC) and may be able to release factors, such as calcitonin gene‐related peptide and epinephrine, that affect LC function. LC and the LC‐like cell line XS106 express mRNA for the pituitary adenylate cyclase‐activating polypeptide (PACAP)...

Full description

Saved in:
Bibliographic Details
Published in:European journal of immunology 2003-11, Vol.33 (11), p.3070-3079
Main Authors: Kodali, Sreedevi, Friedman, Ilyse, Ding, Wanhong, Seiffert, Kristina, Wagner, John A., Granstein, Richard D.
Format: Article
Language:English
Subjects:
Animals
Antigen presentation
Antigens, CD - biosynthesis
Antigens, CD - genetics
B7-2 Antigen
Dermatitis, Contact - immunology
Dermatitis, Contact - metabolism
Hybridomas - immunology
Interleukin-10 - immunology
Langerhans cells
Langerhans Cells - immunology
Membrane Glycoproteins - biosynthesis
Membrane Glycoproteins - genetics
Mice
Neuropeptides
Neuropeptides - metabolism
Pituitary Adenylate Cyclase-Activating Polypeptide
Skin
Skin - immunology
Skin - metabolism
T-Lymphocytes - immunology
VPAC1 protein
VPAC2 protein
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Epidermal nerves are closely associated with Langerhans cells (LC) and may be able to release factors, such as calcitonin gene‐related peptide and epinephrine, that affect LC function. LC and the LC‐like cell line XS106 express mRNA for the pituitary adenylate cyclase‐activating polypeptide (PACAP) receptors VPAC1 and VPAC2. We examined whether PACAP regulates cutaneous immunity. Intradermal administration of PACAP prior to application of a contact sensitizer at the injected site inhibited the induction of contact hypersensitivity. Pretreatment of murine epidermal cells enriched for LC content (∼12% LC) with PACAP inhibited their ability to elicit delayed‐type hypersensitivity in previously immunized mice. In vitro, PACAP suppressed the ability of both murine epidermal cells and highly purified LC (∼95%) to present antigen to a T cell clone and hybridoma. Furthermore, in LC and the XS106 cell line, PACAP inhibited the LPS/GM‐CSF‐induced stimulation of IL‐1β secretion and augmented IL‐10 production. PACAP also down‐regulated CD86 expression in LPS/GM‐CSF‐stimulated XS106 cells. The immunosuppressive effects of PACAP may be due to modulation ofcytokine production and CD86 expression.
ISSN:0014-2980
1521-4141
DOI:10.1002/eji.200324085