Cholesterol-dependent infection of Burkitt's lymphoma cell lines by Epstein-Barr virus

Department of Microbiology-Immunology, Northwestern University Medical School Chicago, IL 60611, USA Correspondence Richard Longnecker r-longnecker{at}northwestern.edu Epstein–Barr virus (EBV) infection is a multi-step process, first requiring virus binding to the host cell, followed by fusion of th...

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Bibliographic Details
Published in:Journal of general virology 2003-11, Vol.84 (11), p.2987-2992
Main Authors: Katzman, Rebecca B, Longnecker, Richard
Format: Article
Language:English
Subjects:
beta-Cyclodextrins
Burkitt Lymphoma - virology
Cholesterol - physiology
Cyclodextrins - pharmacology
Epstein-Barr virus
Herpesvirus 4, Human - physiology
Humans
lipid rafts
Membrane Glycoproteins - physiology
Membrane Microdomains - physiology
methyl-^b-cyclodextrin
Nystatin - pharmacology
Signal Transduction
Tumor Cells, Cultured
Viral Envelope Proteins - physiology
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Summary:Department of Microbiology-Immunology, Northwestern University Medical School Chicago, IL 60611, USA Correspondence Richard Longnecker r-longnecker{at}northwestern.edu Epstein–Barr virus (EBV) infection is a multi-step process, first requiring virus binding to the host cell, followed by fusion of the viral envelope with the host cell plasma membrane. Efficient EBV entry into B cells requires, at the minimum, the interaction of the EBV-encoded glycoproteins gp350 with cellular CD21 and gp42 with MHC class II proteins. In this study, use of the cholesterol-binding drugs methyl- -cyclodextrin and nystatin efficiently inhibited EBV infection of target Burkitt's lymphoma B-cell lines, indicating an important role for cholesterol and suggesting the involvement of lipid rafts in EBV infection.
ISSN:0022-1317
1465-2099
DOI:10.1099/vir.0.19252-0