Improvement of Insulin Absorption from Intratracheally Administrated Dry Powder Prepared by Supercritical Carbon Dioxide Process

• Published in: Journal of pharmaceutical sciences 2003-12, Vol.92 (12), p.2475-2486
• Main Authors: Todo, Hiroaki, Iida, Kotaro, Okamoto, Hirokazu, Danjo, Kazumi
• Format: Article
• Language: English
• Subjects: Absorption - drug effects; Animals; Area Under Curve; Biological and medical sciences; Carbon Dioxide; Cattle; Chromatography, Supercritical Fluid - instrumentation; Chromatography, Supercritical Fluid - methods; General pharmacology; Hormones. Endocrine system; Injections, Spinal; insulin; Insulin - administration & dosage; Insulin - chemical synthesis; Insulin - pharmacokinetics; Male; Medical sciences; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; powder technology; Powders; pulmonary drug delivery; Rats; Rats, Sprague-Dawley; spray drying; supercritical fluids
• ISSN: 0022-3549; 1520-6017
• DOI: 10.1002/jps.10497

The purpose of this study was to improve insulin absorption from dry powder after administration in lung without an absorption enhancer. The dry powders, with mannitol as a carrier, were prepared with or without an absorption enhancer (citric acid) by supercritical carbon dioxide (SCF) and spray drying (SD) processes. Insulin powder was precipitated from dimethyl sulfoxide and aqueous solutions by dispersing the insulin solutions from parallel and V‐type nozzles, respectively, into supercritical carbon dioxide, which is an antisolvent for insulin. In vitro aerosol performance was evaluated with a cascade impactor. Insulin powder containing citric acid prepared by the SCF method (MIC SCF) showed improved inhalation performance compared with insulin powder prepared by the SD process, although the particle size of the former powder was larger than that in powders prepared by SD. Insulin absorption was estimated from the change in plasma glucose level. The blood glucose level after administration of the insulin powder without citric acid prepared by the SCF process (MI SCF) decreased rapidly, and a significant difference was observed for areas under the curve of change in plasma glucose concentration versus time (AUCs) between MI SCF and the insulin powder without citric acid prepared by the SD process (MI SD). These results suggest that the SCF technique would be useful to prepare dry powders suitable for inhalation. © 2003 Wiley‐Liss, Inc. and the American Pharmacists Association J Pharm Sci 92:2475–2486, 2003