Changes in tissue cellularity are associated with growth enhancement in genetically modified arctic char (Salvelinus alpinus L.) carrying recombinant growth hormone gene

Biochemical and histological analyses were used to study the number and size of cells (cellularity) in tissues of fast-growing, genetically modified Arctic char (Salvelinus alpinus L.), overexpressing sockeye salmon (Oncorhynchus nerka) growth hormone gene (OnGH1). DNA contents of muscle, heart, and...

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Bibliographic Details
Published in:Marine biotechnology (New York, N.Y.) N.Y.), 2001-03, Vol.3 (2), p.188-197
Main Authors: Pitkänen, T I, Xie, S Q, Krasnov, A, Mason, P S, Mölsä, H, Stickland, N C
Format: Article
Language:English
Subjects:
Brackish
Cell growth
Cell proliferation
Cell size
Cells
Control
Deoxyribonucleic acid
DNA
Fish
Freshwater
Freshwater fishes
Genetic modification
Growth hormones
Heart
Hormones
Hyperplasia
Hypertrophy
Liver
Marine
Nuclei
Nucleus
Oncorhynchus nerka
OnGH1 gene
Proliferation
Recombinants
Salmon
Salvelinus alpinus
Tissue
Tissues
transgenic fish
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Summary:Biochemical and histological analyses were used to study the number and size of cells (cellularity) in tissues of fast-growing, genetically modified Arctic char (Salvelinus alpinus L.), overexpressing sockeye salmon (Oncorhynchus nerka) growth hormone gene (OnGH1). DNA contents of muscle, heart, and liver were compared in transformed, sibling (age control) and 1 year older (size control) char. Total white muscle cross-sectional area, white muscle fiber number, and total nuclei number within the muscle tissue were determined from one complete half-section of each fish. The analyzed tissues responded differently to growth hormone overproduction. In muscle tissue of OnGH1-transformed char, the enhanced growth was clearly associated with proliferation of muscle cells (hyperplasia), whereas in heart tissue both cell proliferation and increase in cell size (hypertrophy) were enhanced. The relative DNA concentration in the liver of transformed char was significantly greater than that of control fish, suggesting reduction in size of hepatic cells.
ISSN:1436-2228
1436-2236
DOI:10.1007/s101260000060