Helicobacter pylori infection influences expression of genes related to angiogenesis and invasion in human gastric carcinoma cells

Infection with Helicobacter pylori ( H. pylori) is considered a risk factor for gastric carcinoma. The purpose of this study was to clarify whether H. pylori infection plays a role in progression of gastric carcinoma. We examined the expression of genes encoding angiogenic factors and proteases by h...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2003-11, Vol.311 (4), p.809-814
Main Authors: Kitadai, Yasuhiko, Sasaki, Atsunori, Ito, Masanori, Tanaka, Shinji, Oue, Naohide, Yasui, Wataru, Aihara, Miki, Imagawa, Kenichi, Haruma, Ken, Chayama, Kazuaki
Format: Article
Language:English
Subjects:
Adenocarcinoma - genetics
Adenocarcinoma - metabolism
Adenocarcinoma - pathology
Angiogenesis
Angiogenesis Inducing Agents - metabolism
Carcinoma, Adenosquamous - genetics
Carcinoma, Adenosquamous - metabolism
Carcinoma, Adenosquamous - pathology
cDNA microarray
Cell Line, Tumor
Collagenases - biosynthesis
Endopeptidases - genetics
Gastric carcinoma
Gene Expression Regulation, Bacterial - genetics
Helicobacter Infections - complications
Helicobacter Infections - genetics
Helicobacter pylori
Helicobacter pylori - genetics
Humans
Invasion
Neovascularization, Pathologic - genetics
Stomach Neoplasms - etiology
Stomach Neoplasms - genetics
Stomach Neoplasms - metabolism
Stomach Neoplasms - pathology
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Summary:Infection with Helicobacter pylori ( H. pylori) is considered a risk factor for gastric carcinoma. The purpose of this study was to clarify whether H. pylori infection plays a role in progression of gastric carcinoma. We examined the expression of genes encoding angiogenic factors and proteases by human gastric carcinoma cell lines (MKN-1 and TMK-1) co-cultured with or without H. pylori by cDNA microarray analysis. Co-culture with H. pylori increased expression of mRNAs encoding interleukin (IL)-8, vascular endothelial growth factor (VEGF), angiogenin, urokinase-type plasminogen activator (uPA), and metalloproteinase (MMP)-9 by gastric carcinoma cells. Up-regulation of these genes at the mRNA and protein levels was confirmed by Northern blot analysis, semi-quantitative RT-PCR analysis, and ELISA. In vitro angiogenic and collagenase activities of conditioned medium from the gastric carcinoma cells were also stimulated by co-culture with H. pylori. These results indicate that H. pylori infection may regulate angiogenesis and invasion of human gastric carcinoma.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2003.10.077