DMSO as a vehicle for central injections: tests with feeding elicited by norepinephrine injected into the paraventricular nucleus

Dimethyl sulfoxide (DMSO) is becoming increasingly popular as a vehicle in studies employing central injections. The aim of the present study was to determine whether the vehicle required for solubilization of substances for central injection [75% DMSO and 25% artificial CSF (aCSF)] would alter the...

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Bibliographic Details
Published in:Pharmacology, biochemistry and behavior biochemistry and behavior, 2002-01, Vol.71 (1), p.277-282
Main Authors: Blevins, J.Ernie, Stanley, B.Glenn, Reidelberger, Roger D.
Format: Article
Language:English
Subjects:
Animals
Appetite
Biological and medical sciences
Brain
Cerebrospinal Fluid - physiology
Dimethyl Sulfoxide - administration & dosage
Eating - drug effects
Eating - physiology
General pharmacology
Injections, Intraventricular
Male
Medical sciences
Neuropeptide
Norepinephrine - administration & dosage
Norepinephrine - cerebrospinal fluid
Paraventricular Hypothalamic Nucleus - drug effects
Paraventricular Hypothalamic Nucleus - physiology
Pharmaceutical technology. Pharmaceutical industry
Pharmaceutical Vehicles - administration & dosage
Pharmacology. Drug treatments
Rats
Rats, Sprague-Dawley
Regulation
Satiety
Solvents - administration & dosage
Sympathomimetics - administration & dosage
Sympathomimetics - cerebrospinal fluid
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Summary:Dimethyl sulfoxide (DMSO) is becoming increasingly popular as a vehicle in studies employing central injections. The aim of the present study was to determine whether the vehicle required for solubilization of substances for central injection [75% DMSO and 25% artificial CSF (aCSF)] would alter the well-characterized stimulatory response to norepinephrine (NE) injected into the paraventricular nucleus (PVN) on short-term food intake. To evaluate its suitability, we compared the effects of repeated unilateral injections of NE dissolved in two different vehicles (100% aCSF or 75% DMSO, 25% aCSF), in separate groups of animals every 48 h over a 30-day period. NE (40 nmol) stimulated food intake by approximately sevenfold compared to either vehicle alone, and the stimulatory effect was similar whether aCSF or 75% DMSO was used as a vehicle. Furthermore, the NE-induced feeding did not vary in magnitude across a series of 13 tests. These results suggest that 75% DMSO is a suitable vehicle for administering NE (and likely other water-insoluble substances)in small volumes of 0.3 μl into specific brain regions.
ISSN:0091-3057
1873-5177
DOI:10.1016/S0091-3057(01)00659-1