Complex alternative processing of human cytomegalovirus UL37 pre-mRNA

1 Center for Cancer and Immunology Research, Children's Research Institute, Children's National Medical Center, George Washington University School of Medicine and Health Sciences, 111 Michigan Avenue, NW, Washington, DC 20010, USA 2 Department of Pediatrics, George Washington University S...

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Bibliographic Details
Published in:Journal of general virology 2003-12, Vol.84 (12), p.3353-3358
Main Authors: Adair, Richard, Liebisch, Gregory W, Colberg-Poley, Anamaris M
Format: Article
Language:English
Subjects:
Alternative Splicing
Amino Acid Sequence
AUL37 gene
Cells, Cultured
DNA, Complementary - analysis
Human cytomegalovirus
Humans
Immediate-Early Proteins - genetics
Immediate-Early Proteins - metabolism
Molecular Sequence Data
Polymerase Chain Reaction
RNA, Messenger - biosynthesis
RNA, Viral - biosynthesis
UL37 gene
Viral Proteins
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Summary:1 Center for Cancer and Immunology Research, Children's Research Institute, Children's National Medical Center, George Washington University School of Medicine and Health Sciences, 111 Michigan Avenue, NW, Washington, DC 20010, USA 2 Department of Pediatrics, George Washington University School of Medicine and Health Sciences, 111 Michigan Avenue, NW, Washington, DC 20010, USA Correspondence Anamaris M. Colberg-Poley acolberg-poley{at}cnmcresearch.org Differentially processed human cytomegalovirus (HCMV) UL37 RNAs encode biologically significant proteins. Due to the recent discovery of alternative UL37 exon 3 (UL37x3) splice donors, permissively infected cells were thoroughly examined for additional alternatively spliced UL37 RNAs. Newly described donors within UL37 exon 1 (nt 52520) and intron 1 (nt 52209) as well as UL37x3 di (nt 50770) and dii (nt 50782) were differentially spliced to known downstream UL37 acceptors. The alternatively spliced UL37 S , UL37 L , UL37 di and UL37d ii RNAs predictably encode proteins of 83, 163, 217 and 213 residues, respectively, which share UL37x1 N-terminal sequences but differ downstream in their C termini. Moreover, temporal expression of the alternatively spliced UL37 RNAs differs during HCMV infection. The complexity of UL37 pre-mRNA processing is evidenced by the detection of 11 UL37 spliced and unspliced UL37x1 RNAs in HCMV-infected cells. Based upon these data, a revised HCMV UL37 gene map is presented, which incorporates all RNA species detected during permissive infection.
ISSN:0022-1317
1465-2099
DOI:10.1099/vir.0.19404-0