Naturally occurring human autoantibodies recognize a fetal brain antigen identified as microtubulus associated Protein 1B

We have recently reported naturally occurring autoantibodies against a large fetal brain antigen (FBA). Now we describe the process of purification and identification of this particular FBA. The brains of newborn rabbits were solubilized and purified with preparative gel electrophoresis. The protein...

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Bibliographic Details
Published in:Life sciences (1973) 2002-01, Vol.70 (8), p.887-892
Main Authors: Peltola, Jukka, Hellman, Jukka, Hakkarainen, Kati, Marttinen, Aulis, Soppi, Esa, Molnar, Gabor K.
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Antibodies, Monoclonal
Autoantibodies - immunology
Autoantigens - immunology
Autoantigens - isolation & purification
Brain - immunology
Chromatography, High Pressure Liquid
Electrophoresis, Polyacrylamide Gel
Fetal brain antigens
Fetus
Humans
Immunoblotting
Microtubule-Associated Proteins - immunology
Microtubule-Associated Proteins - isolation & purification
Microtubulus associated protein 1B
Naturally occurring autoantibodies
Rabbits
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Summary:We have recently reported naturally occurring autoantibodies against a large fetal brain antigen (FBA). Now we describe the process of purification and identification of this particular FBA. The brains of newborn rabbits were solubilized and purified with preparative gel electrophoresis. The protein fractions were concentrated and desalted and the fractions were tested by a known positive serum. On membrane digestion of the FBA-band gave a twelve amino acid sequence that resulted in best identity score for mouse, rat and human microtubule-associated protein (MAP) 1B; a member of the microtubule-associated protein family. Monoclonal anti-MAP1B recognized a band in immunoblots of the brain homogenate and of the partially purified fractions with the same electrophoretic mobility as that recognized by a known anti-FBA positive serum. When adult rabbit brain was used as an antigen, the anti-MAP1B failed to recognize any bands on immunoblots. MAP 1B has not been previously known as an autoantigen, even though many structural proteins of the neuronal cytoskeleton are known to be targets of naturally occurring autoantibodies. MAP 1B is a functionally important regulatory protein in the developing brain; thus autoantibodies against MAP1B may affect the normal development.
ISSN:0024-3205
1879-0631
DOI:10.1016/S0024-3205(01)01462-X