Identification and characterization of novel calcium‐binding proteins of Dictyostelium and their spatial expression patterns during development

Five putative Ca2+‐binding proteins, CBP5, 6, 7, 8 and 9, all having EF‐hand motifs, were found by searching the Dictyostelium cDNA database (http://www.csm.biol.tsukuba.ac.jp/cDNAproject.html). 45Ca2+‐overlay experiments revealed that four of these (excluding CBP9) are real Ca2+‐binding proteins. N...

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Published in:Development, growth & differentiation growth & differentiation, 2003-10, Vol.45 (5‐6), p.507-514
Main Authors: Sakamoto, Haruyo, Nishio, Keiko, Tomisako, Mariko, Kuwayama, Hidekazu, Tanaka, Yoshimasa, Suetake, Isao, Tajima, Shouji, Ogihara, Satoshi, Coukell, Barrie, Maeda, Mineko
Format: Article
Language:English
Subjects:
Amino Acid Motifs
Amino Acid Sequence
Animals
Blotting, Northern
Ca2+‐binding proteins
Calcium - chemistry
Calcium - metabolism
Calcium-Binding Proteins - metabolism
Cell Differentiation
Cell Line
Databases as Topic
Dictyostelium
Dictyostelium - cytology
DNA, Complementary - metabolism
Gene Expression Regulation, Developmental
Glutathione Transferase - metabolism
In Situ Hybridization
Molecular Sequence Data
morphogenesis
Phylogeny
Recombinant Fusion Proteins - metabolism
Sequence Homology, Amino Acid
spatial expression patterns
Time Factors
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Summary:Five putative Ca2+‐binding proteins, CBP5, 6, 7, 8 and 9, all having EF‐hand motifs, were found by searching the Dictyostelium cDNA database (http://www.csm.biol.tsukuba.ac.jp/cDNAproject.html). 45Ca2+‐overlay experiments revealed that four of these (excluding CBP9) are real Ca2+‐binding proteins. Northern blot analysis revealed that the genes encoding CBP5, 6, 7 and 8 are all developmentally regulated. In situ hybridization analyses revealed that spatial expression of these genes was regulated in several different ways. CBP1, 2, 3, 5, 6 and 7 are expressed in prespore cells in the slug stage. Transcripts of the genes for CBP1 and 5 are enriched in prestalk subtype PstO cells. In contrast, CBP4 is expressed predominantly in PstO cells. CBP8 is evenly expressed at a very low level throughout the whole slug. Such distinct spatial expression patterns suggest that the CBP might be involved in morphogenesis and might have their own roles either in prespore or in prestalk cell differentiation of Dictyostelium.
ISSN:0012-1592
1440-169X
DOI:10.1111/j.1440-169X.2003.00718.x