YB-1 Relocates to the Nucleus in Adenovirus-infected Cells and Facilitates Viral Replication by Inducing E2 Gene Expression through the E2 Late Promoter

The adenovirus early proteins E1A and E1B-55kDa are key regulators of viral DNA replication, and it was thought that targeting of p53 by E1B-55kDa is essential for this process. Here we have identified a previously unrecognized function of E1B for adenovirus replication. We found that E1B-55kDa is i...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of biological chemistry 2002-03, Vol.277 (12), p.10427-10434
Main Authors: Holm, Per S., Bergmann, Stephan, Jürchott, Karsten, Lage, Hermann, Brand, Karsten, Ladhoff, Axel, Mantwill, Klaus, Curiel, David T., Dobbelstein, Matthias, Dietel, Manfred, Gänsbacher, Bernd, Royer, Hans-Dieter
Format: Article
Language:English
Subjects:
Active Transport, Cell Nucleus
Adenoviridae - genetics
Adenoviridae - metabolism
Adenovirus
Adenovirus E2 Proteins - genetics
Antigens, Bacterial
Bacterial Proteins - metabolism
Blotting, Northern
Blotting, Southern
Blotting, Western
CCAAT-Enhancer-Binding Proteins - metabolism
Cell Line
Cell Nucleus - metabolism
DNA, Complementary - metabolism
DNA-Binding Proteins
E2 protein
Gene Expression Regulation
HeLa Cells
Humans
Microscopy, Confocal
Microscopy, Electron
Microscopy, Fluorescence
NFI Transcription Factors
Nuclear Proteins
Promoter Regions, Genetic
Protein Binding
Transcription Factors
Transgenes
Virus Replication
Y-Box-Binding Protein 1
YB-1 protein
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The adenovirus early proteins E1A and E1B-55kDa are key regulators of viral DNA replication, and it was thought that targeting of p53 by E1B-55kDa is essential for this process. Here we have identified a previously unrecognized function of E1B for adenovirus replication. We found that E1B-55kDa is involved in targeting the transcription factor YB-1 to the nuclei of adenovirus type 5-infected cells where it is associated with viral inclusion bodies believed to be sites of viral transcription and replication. We show that YB-1 facilitates E2 gene expression through the E2 late promoter thus controlling E2 gene activity at later stages of infection. The role of YB-1 for adenovirus replication was demonstrated with an E1-minus adenovirus vector containing a YB-1 transgene. In infected cells, AdYB-1 efficiently replicated and produced infectious progeny particles. Thus, adenovirus E1B-55kDa protein and the host cell factor YB-1 act jointly to facilitate adenovirus replication in the late phase of infection.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M106955200