Similar Frequency of Rhinovirus-Infectible Cells in Upper and Lower Airway Epithelium

Rhinovirus (RV) infections can alter lower airway physiology and inflammation, yet the characteristics of RV replication in lower airway cells are incompletely understood. An RV serotype 16 (RV16)-specific monoclonal antibody was identified. Immunohistochemistry and an infectious center assay were u...

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Bibliographic Details
Published in:The Journal of infectious diseases 2002-03, Vol.185 (6), p.734-743
Main Authors: Mosser, Anne G., Brockman-Schneider, Rebecca, Amineva, Svetlana, Burchell, Lacinda, Sedgwick, Julie B., Busse, William W., Gern, James E.
Format: Article
Language:English
Subjects:
Adenoids - virology
Antibacterial agents
Antibiotics. Antiinfectious agents. Antiparasitic agents
Antibodies
Bacteriology
Biological and medical sciences
Bronchi - virology
Cultured cells
Epithelial cells
Epithelial Cells - virology
Flow Cytometry
Fundamental and applied biological sciences. Psychology
HeLa Cells
Humans
Immunohistochemistry
Infections
Intercellular Adhesion Molecule-1 - analysis
Isotypes
Medical sciences
Microbiology
Monoclonal antibodies
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Pharmacology. Drug treatments
Photographic slides
Rhinovirus
Rhinovirus - growth & development
Virus Replication
Viruses
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Summary:Rhinovirus (RV) infections can alter lower airway physiology and inflammation, yet the characteristics of RV replication in lower airway cells are incompletely understood. An RV serotype 16 (RV16)-specific monoclonal antibody was identified. Immunohistochemistry and an infectious center assay were used to quantitate the infectivity of RV16 in primary bronchial and adenoidal epithelial cells. The proportion of infectible epithelial cells increased with the inoculum but did not exceed 10%. Analysis of bronchial tissue samples infected ex vivo demonstrated a small subset of RV-infected cells in the epithelial layer. These data confirm previous reports that RV infects only a small subset of epithelial cells in upper airway tissues and indicate that lower airway epithelial cells have a similar susceptibility to RV infection. In confirming that RV can infect cells in the lower airway, these results suggest that lower airway dysfunction occurs through this mechanism in susceptible persons.
ISSN:0022-1899
1573-6613
1537-6613
DOI:10.1086/339339