In vitro and in vivo transfection efficiency of a novel ultradeformable cationic liposome

Cationic lipids have been often used as one of the major components in making most promising non-viral gene delivery systems, whereas sodium cholate, a surfactant so-called edge activator has been used in preparing ultradeformable and ultraflexible liposomes called Transfersomes. Using both a cation...

Full description

Saved in:
Bibliographic Details
Published in:Biomaterials 2004, Vol.25 (2), p.305-313
Main Authors: Kim, Adele, Lee, Eun Hye, Choi, Sung-Hee, Kim, Chong-Kook
Format: Article
Language:English
Subjects:
Cationic-lipids
DNA - metabolism
Fatty Acids, Monounsaturated - metabolism
Genes, Reporter
Humans
Liposomes - metabolism
Non-invasive delivery
Quaternary Ammonium Compounds - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA - isolation & purification
Skin
Sodium Cholate - metabolism
Time Factors
Transfection
Transfersomes
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cationic lipids have been often used as one of the major components in making most promising non-viral gene delivery systems, whereas sodium cholate, a surfactant so-called edge activator has been used in preparing ultradeformable and ultraflexible liposomes called Transfersomes. Using both a cationic lipid, DOTAP and sodium cholate, a novel formulation of ultradeformable cationic liposome (UCL) has been prepared. The average particle size of this formulation was approximately 80 nm. The physical and chemical stabilities at two different temperatures (4°C and 20°C) were also evaluated for 60 days. The ultradeformability of new formulation was also assessed, and it has been proved that the formulation is deformable. In vitro transfection efficiency of plasmid DNA/UCL was assessed by the expression of green fluorescent protein (GFP) in four cell lines, OVCAR-3 (human ovarian carcinoma cells), HepG2 (human hepatoma cells), H-1299 (human lung carcinoma cells) and T98G (human brain carcinoma cells). The optimal ratio of DNA to liposome for maximal transfection efficiency was 1:14 (w/w) in all the cell lines except for the human brain carcinoma cells. The same formulation was tested for in vivo transfection efficiency and its retention time within the organs by applying the DNA/UCL complexes on hair-removed dorsal skin of mice non-invasively. It was found that genes were transported into several organs for 6 days once applied on intact skin.
ISSN:0142-9612
1878-5905
DOI:10.1016/S0142-9612(03)00534-9