Albendazole sulphoxide concentrations in plasma of endemic normals from a lymphatic filariasis endemic region using liquid chromatography

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2004-01, Vol.799 (2), p.233-238
• Main Authors: Sarin, Reema, Dash, A.P, Dua, V.K
• Format: Article
• Language: English
• Subjects: Adult; Albendazole - analogs & derivatives; Albendazole - blood; Albendazole - pharmacokinetics; Albendazole sulphoxide; Analysis; Analytical, structural and metabolic biochemistry; Anthelmintics - blood; Anthelmintics - pharmacokinetics; Biological and medical sciences; Calibration; Chromatography, High Pressure Liquid - methods; Fundamental and applied biological sciences. Psychology; General pharmacology; Humans; Medical sciences; Pharmacology. Drug treatments; Reference Standards; Reference Values; Reproducibility of Results; Sensitivity and Specificity; Spectrophotometry, Ultraviolet
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2003.10.040

A simple and sensitive reversed-phase isocratic HPLC method for the determination of albendazole and its metabolites has been developed. The mobile phase consisting of acetonitrile–water–perchloric acid (70%) (30:110:0.06 (v/v/v)) was pumped at a flow rate of 0.80 ml/min on a 5 μm, reverse phase, Discovery ® RPamide C16 column with UV detection at 290 nm. The calibration graphs were linear in the range of 0.05–1 μg/ml for albendazole, albendazole sulphoxide and albendazole sulphone. The limit of quantification was 50 ng/ml for albendazole, 25 ng/ml for albendazole sulphoxide and 30 ng/ml for albendazole sulphone. The within-day and day-to-day coefficient of variation averaged 4.98 and 6.95% for albendazole, 3.83 and 6.83% for albendazole sulphoxide and 3.44 and 5.51% for albendazole sulphone, respectively. The mean extraction recoveries of albendazole, albendazole sulphoxide and albendazole sulphone were 79.25, 93.03 and 88.78%, respectively. The method was applied to determine the plasma levels of albendazole sulphoxide in endemic normals administered with albendazole during pharmacokinetic studies.