Functional Annotation of a Full-Length Arabidopsis cDNA Collection

Full-length complementary DNAs (cDNAs) are essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. We isolated 155,144 RIKEN Arabidopsis full-length (RAFL) cDNA clones. The 3′-end expressed sequence tags (ESTs) of 155,144 RAFL cDNAs were...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2002-04, Vol.296 (5565), p.141-145
Main Authors: SEKI, Motoaki, NARUSAKA, Mari, MURAMATSU, Masami, HAYASHIZAKI, Yoshihide, KAWAI, Jun, CARNINCI, Piero, ITOH, Masayoshi, ISHII, Yoshiyuki, ARAKAWA, Takahiro, SHIBATA, Kazuhiro, SHINAGAWA, Akira, SHINOZAKI, Kazuo, KAMIYA, Asako, ISHIDA, Junko, SATOU, Masakazu, SAKURAI, Tetsuya, NAKAJIMA, Maiko, ENJU, Akiko, AKIYAMA, Kenji, OONO, Youko
Format: Article
Language:English
Subjects:
Arabidopsis
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Arabidopsis thaliana
Biological and medical sciences
Botany
CDNA libraries
Chromosome Mapping
Cloning
Cloning, Molecular
Complementary DNA
Computational Biology
Databases, Nucleic Acid
Deoxyribonucleic acid
Developmental Stages
DNA
DNA, Complementary - isolation & purification
DNA, Plant - genetics
Expressed Sequence Tags
Flowers & plants
Fundamental and applied biological sciences. Psychology
Gene Expression
Gene Library
Genes
Genes, Plant
Genes. Genome
Genetic aspects
Genome, Plant
Genomes
Genomics
Libraries
Molecular and cellular biology
Molecular genetics
Plant biotechnology
Plants
Promoter Regions, Genetic
Proteins
RNA, Messenger - genetics
Sequence Analysis, DNA
Sequencing
Telomeres
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Description
Summary:Full-length complementary DNAs (cDNAs) are essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. We isolated 155,144 RIKEN Arabidopsis full-length (RAFL) cDNA clones. The 3′-end expressed sequence tags (ESTs) of 155,144 RAFL cDNAs were clustered into 14,668 nonredundant cDNA groups, about 60% of predicted genes. We also obtained 5′ ESTs from 14,034 nonredundant cDNA groups and constructed a promoter database. The sequence database of the RAFL cDNAs is useful for promoter analysis and correct annotation of predicted transcription units and gene products. Furthermore, the full-length cDNAs are useful resources for analyses of the expression profiles, functions, and structures of plant proteins.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1071006