Atomic force microscopy evaluation of aqueous interfaces of immobilized hyaluronan

Hyaluronan (HA) was immobilized on aminated glass surfaces in three different ways: by simple ionic interaction and by covalent linking at low density and at full density. In agreement with previous reports, in vitro experiments show that the outcome of fibroblast adhesion tests is markedly affected...

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Bibliographic Details
Published in:Journal of colloid and interface science 2003-03, Vol.259 (2), p.236-243
Main Authors: Morra, Marco, Cassinelli, Clara, Pavesio, Alessandra, Renier, Davide
Format: Article
Language:English
Subjects:
Animals
Atomic force microscope
Biological and medical sciences
Biomaterials
Cell Adhesion
Fibroblasts - chemistry
Glass - chemistry
Hyaluronan
Hyaluronic Acid - chemistry
Medical sciences
Mice
Microscopy, Atomic Force
Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)
Surface analysis
Surface modification
Technology. Biomaterials. Equipments. Material. Instrumentation
Water - chemistry
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Summary:Hyaluronan (HA) was immobilized on aminated glass surfaces in three different ways: by simple ionic interaction and by covalent linking at low density and at full density. In agreement with previous reports, in vitro experiments show that the outcome of fibroblast adhesion tests is markedly affected by the details of the coupling procedure, suggesting that different interfacial forces are operating at the aqueous/HA interface in the three cases investigated. The interfacial properties of the HA-coated surfaces were probed by force–distance curves obtained with the atomic force microscope (AFM). This approach readily shows significant differences among the tested samples, which are directly related to the coupling strategy and to results of cell adhesion tests. In particular, the range of interaction between the tip and the surface is much lower when HA is covalently linked than when it is ionically coupled, suggesting a more compact surface structure in the former case. Increasing HA surface density minimizes the interaction force between the surface and the AFM tip, likely reflecting more complete shielding by the HA chains of the underlying substrate. In summary, these measurements clearly show the different nature of the aqueous interfaces tested, and underline the role of this analytical approach in the development and control of finely tuned biomaterial surfaces.
ISSN:0021-9797
1095-7103
DOI:10.1016/S0021-9797(02)00204-7