Role Played by Serum, a Biological Cue, in the Adherence of Enterococcus faecalis to Extracellular Matrix Proteins, Collagen, Fibrinogen, and Fibronectin

Background. Most previous studies have found that Enterococcus faecalis isolates do not show significant adherence to fibronectin and fibrinogen. Methods. The influence of various conditions on E. faecalis adherence to extracellular matrix (ECM) proteins was evaluated using a radiolabeled-cell adher...

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Bibliographic Details
Published in:The Journal of infectious diseases 2008-06, Vol.197 (12), p.1728-1736
Main Authors: Nallapareddy, Sreedhar R., Murray, Barbara E.
Format: Article
Language:English
Subjects:
Animals
Bacteria
Bacterial Adhesion
Bacteriology
Biological and medical sciences
Cell growth
Collagen - physiology
Collagens
Endocarditis
Enterococcus faecalis
Enterococcus faecalis - physiology
Extracellular matrix proteins
Extracellular Matrix Proteins - physiology
Fibrinogen - physiology
Fibronectins - physiology
Fundamental and applied biological sciences. Psychology
Horses
Humans
Infections
Infectious diseases
Medical sciences
Microbiology
Miscellaneous
Serum - physiology
Steepest descent method
Time Factors
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Summary:Background. Most previous studies have found that Enterococcus faecalis isolates do not show significant adherence to fibronectin and fibrinogen. Methods. The influence of various conditions on E. faecalis adherence to extracellular matrix (ECM) proteins was evaluated using a radiolabeled-cell adherence assay. Results. Among the conditions studied, growth in 40% horse serum (a biological cue with potential clinical relevance) elicited adherence of all 46 E. faecalis strains tested to fibronectin and fibrinogen but not to elastin; adherence levels were independent of strain source, and adherence was eliminated by treating cells with trypsin. As previously reported, serum also elicited adherence to collagen. Although prolonged exposure to serum during growth was needed for enhancement of adherence to fibrinogen, brief exposure (
ISSN:0022-1899
1537-6613
DOI:10.1086/588143