HPLC and chemometrics-assisted UV-spectroscopy methods for the simultaneous determination of ambroxol and doxycycline in capsule

High-performance liquid chromatography (HPLC) and multivariate spectrophotometric methods are described for the simultaneous determination of ambroxol hydrochloride (AM) and doxycycline (DX) in combined pharmaceutical capsules. The chromatographic separation was achieved on reversed-phase C 18 analy...

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Bibliographic Details
Published in:Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2008-08, Vol.70 (3), p.655-663
Main Authors: Hadad, Ghada M., El-Gindy, Alaa, Mahmoud, Waleed M.M.
Format: Article
Language:English
Subjects:
Ambroxol
Ambroxol - analysis
Analysis of Variance
Calibration
Capsules
Chromatography, High Pressure Liquid - methods
Doxycycline
Doxycycline - analysis
HPLC
Least-Squares Analysis
Multivariate spectrophotometric methods
Pharmaceutical Preparations
Reproducibility of Results
Solutions
Spectrophotometry, Ultraviolet - methods
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Summary:High-performance liquid chromatography (HPLC) and multivariate spectrophotometric methods are described for the simultaneous determination of ambroxol hydrochloride (AM) and doxycycline (DX) in combined pharmaceutical capsules. The chromatographic separation was achieved on reversed-phase C 18 analytical column with a mobile phase consisting of a mixture of 20 mM potassium dihydrogen phosphate, pH 6–acetonitrile in ratio of (1:1, v/v) and UV detection at 245 nm. Also, the resolution has been accomplished by using numerical spectrophotometric methods as classical least squares (CLS), principal component regression (PCR) and partial least squares (PLS-1) applied to the UV spectra of the mixture and graphical spectrophotometric method as first derivative of the ratio spectra ( 1DD) method. Analytical figures of merit (FOM), such as sensitivity, selectivity, analytical sensitivity, limit of quantitation and limit of detection were determined for CLS, PLS-1 and PCR methods. The proposed methods were validated and successfully applied for the analysis of pharmaceutical formulation and laboratory-prepared mixtures containing the two component combination.
ISSN:1386-1425
DOI:10.1016/j.saa.2007.08.016