Distribution of Gonadotropin-Releasing Hormone Neurones in the Chick Forebrain is Independent of Lineage Relationships Among Cells of the Early Nasal Placode

The regulation of reproduction depends upon the successful migration of gonadotropin‐releasing hormone (GnRH) neurones from the nasal placode to the ventral forebrain during embryogenesis. Within the central nervous system (CNS), these neurones migrate to stereotyped, highly reproducible locations i...

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Bibliographic Details
Published in:Journal of neuroendocrinology 2002-03, Vol.14 (3), p.207-212
Main Authors: Silverman, A.-J., Cserjesi, P., Kanter, E.
Format: Article
Language:English
Subjects:
Animals
Cell Line
Chick Embryo - cytology
Chick Embryo - metabolism
Gonadotropin-Releasing Hormone - metabolism
LHRH
neuronal migration
Neurons - cytology
Neurons - metabolism
Prosencephalon - embryology
reproduction
Respiratory System - embryology
retrovirus
Tissue Distribution
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Summary:The regulation of reproduction depends upon the successful migration of gonadotropin‐releasing hormone (GnRH) neurones from the nasal placode to the ventral forebrain during embryogenesis. Within the central nervous system (CNS), these neurones migrate to stereotyped, highly reproducible locations in septal, preoptic and hypothalamic nuclei. We postulated that lineage relationships (descent from a common precursor) might predict the final location of these neurones. To test this hypothesis, a complex retroviral library was used to label dividing cells in the placode and subsequently to identify them by the presence of the alkaline phosphatase marker. GnRH was detected immunocytochemically and lineage relationships determined by single cell polymerase chain reaction and sequencing of the degenerate oligonucleotide component of the retrovirus. GnRH‐positive and GnRH‐negative neurones were confined to the side ipsilateral to the injection; many cells derived from the placode that entered the CNS did not contain GnRH. This precise method of identifying and mapping the progeny of single neurones revealed that GnRH cells in any given area were derived from multiple precursors. This developmental pattern may contribute to assuring that all CNS locations critical to the orchestration of reproductive events will be populated by GnRH neurones.
ISSN:0953-8194
1365-2826
DOI:10.1046/j.0007-1331.2001.00762.x