Whole blood platelet deposition on extracellular matrix under flow conditions in preterm neonatal sepsis

Platelet function in preterm infants with sepsis was evaluated by measuring their adhesion and aggregation properties using the Cone and Plate(let) Analyser. This may lead to earlier detection of bleeding tendency in septic infants. Platelet function was investigated in 54 preterm infants, of whom 3...

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Bibliographic Details
Published in:European journal of pediatrics 2002-05, Vol.161 (5), p.270-274
Main Authors: FINKELSTEIN, Yaron, SHENKMAN, Boris, SIROTA, Lea, VISHNE, Tali H, DARDIK, Rima, VARON, David, LINDER, Nehama
Format: Article
Language:English
Subjects:
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Antigens
Biological and medical sciences
Blood
Blood platelets
Blood Platelets - physiology
Collagen
Emergency and intensive care: neonates and children. Prematurity. Sudden death
Extracellular matrix
Extracellular Matrix - metabolism
Female
Gestational Age
Hemorheology
Humans
Infant, Newborn
Infant, Premature - physiology
Intensive care medicine
Male
Medical sciences
Newborn babies
Platelet Adhesiveness - physiology
Platelet Aggregation - physiology
Premature babies
Premature birth
Sepsis
Sepsis - blood
Sepsis - physiopathology
Shear stress
Thrombocytopenia
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Summary:Platelet function in preterm infants with sepsis was evaluated by measuring their adhesion and aggregation properties using the Cone and Plate(let) Analyser. This may lead to earlier detection of bleeding tendency in septic infants. Platelet function was investigated in 54 preterm infants, of whom 32 had proven neonatal sepsis and 22 were healthy matched controls. Citrated whole blood was subjected to shear stress (1300 s(-1)) for 2 min on tissue culture plates precoated with subendothelial extracellular matrix (ECM). The percentage of ECM surface covered with platelets and the average size of the ECM-bound platelet particles were determined with an image analyser. Assays for von Willebrand factor (vWF) antigen, ristocetin co-factor, and vWF collagen binding activity (CBA) were performed on samples from an additional 47 preterm infants: 38 healthy and 9 septic. Platelets of the preterm infants with sepsis displayed lower adhesion than those of the healthy controls. Mean surface coverage was 16.9+/-8.2% for the septic infants, 15.4+/-7.9% for the septic infants after exclusion of those with coagulase-negative staphylococci sepsis, and 20.8+/-9.6% for the healthy group ( P
ISSN:0340-6199
1432-1076
DOI:10.1007/s00431-002-0938-4