Apoptosis Induced in an Early Step of African Swine Fever Virus Entry into Vero Cells Does Not Require Virus Replication

Permissive Vero cells develop apoptosis, as characterized by DNA fragmentation, caspases activation, cytosolic release of mitochondrial cytochrome c, and flow cytometric analysis of DNA content, upon infection with African swine fever virus (ASFV). To determine the step in virus replication that tri...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2002-03, Vol.294 (2), p.372-382
Main Authors: Carrascosa, Angel L., Bustos, Marı́a J., Nogal, Marı́a L., González de Buitrago, Gonzalo, Revilla, Yolanda
Format: Article
Language:English
Subjects:
African swine fever virus
African Swine Fever Virus - physiology
Animals
Apoptosis
caspases
Cercopithecus aethiops
chloroquine
cycloheximide
cytosine arabinoside
Swine
Vero Cells
Viral Proteins - biosynthesis
Virus Replication - physiology
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Summary:Permissive Vero cells develop apoptosis, as characterized by DNA fragmentation, caspases activation, cytosolic release of mitochondrial cytochrome c, and flow cytometric analysis of DNA content, upon infection with African swine fever virus (ASFV). To determine the step in virus replication that triggers apoptosis, we used UV-inactivated virus, inhibitors of protein and nucleic acid synthesis, and lysosomotropic drugs that block virus uncoating. ASFV-induced apoptosis was accompanied by caspase-3 activation, which was detected even in the presence of either cytosine arabinoside or cycloheximide, indicating that viral DNA replication and protein synthesis were not required to activate the apoptotic process. The activation of caspase-3 was released from chloroquine inhibition 2 h after virus absorption, while the infection with UV-inactivated ASFV did not induce the activation of the caspase cascade. We conclude that ASFV induces apoptosis in the infected cell by an intracellular pathway probably triggered during the process of virus uncoating.
ISSN:0042-6822
1096-0341
DOI:10.1006/viro.2001.1348