The control of intracellular glycerol in Saccharomyces cerevisiae influences osmotic stress response and resistance to increased temperature

• Published in: Molecular microbiology 2000-06, Vol.36 (6), p.1381-1390
• Main Authors: Siderius, Marco, Van Wuytswinkel, Olivier, Reijenga, Karin A., Kelders, Marco, Mager, Willem H.
• Format: Article
• Language: English
• Subjects: FPS1 gene; Glyceraldehyde-3-Phosphate Dehydrogenases - genetics; Glycerol - metabolism; GPD1 gene; GPD2 gene; HOG1 gene; Intracellular Fluid - metabolism; Mitogen-Activated Protein Kinases - genetics; Mitogen-Activated Protein Kinases - physiology; Osmotic Pressure; Phosphoric Monoester Hydrolases - genetics; Saccharomyces cerevisiae; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae - physiology; Saccharomyces cerevisiae Proteins; Temperature
• ISSN: 0950-382X; 1365-2958
• DOI: 10.1046/j.1365-2958.2000.01955.x

Glycerol has been demonstrated to serve as the major osmolyte of Saccharomyces cerevisiae. Consistently, mutant strains gpd1gpd2 and gpp1gpp2, which are devoid of the main glycerol biosynthesis pathway, have been shown to be osmosensitive. In addition, the primary hyperosmotic stress response is affected in these strains. Hog1p phosphorylation turned out to be prolonged and osmostress‐induced gene expression is delayed compared with the kinetics observed in wild‐type cells. A hog1 deletion strain was previously found to contain lower internal glycerol and therefore displays an osmosensitive phenotype. Here, we show that the osmosensitivity of hog1 is suppressed by growth at 37°C. We reasoned that this temperature‐remedial osmoresistance might be caused by a higher intracellular glycerol level at the elevated temperature. This hypothesis was confirmed by measurement of the glycerol concentration, which was shown to be similar for wild type and hog1 cells only at elevated growth temperatures. In agreement with this finding, hog1 cells containing an fps1 allele, encoding a constitutively open glycerol channel, have lost their temperature‐remedial osmoresistance. Furthermore, gpd1gpd2 and gpp1gpp2 strains were found to be temperature sensitive. The growth defect of these strains could be suppressed by adding external glycerol. In conclusion, the ability to control glycerol levels influences proper osmostress‐induced signalling and the cellular potential to grow at elevated temperatures. These data point to an important, as yet unidentified, role of glycerol in cellular functioning.