Hemolysate activates P21RAS in rabbit basilar artery

Cerebral vasospasm is the major factor of mortality and morbidity in the patients who have an aneurysmal subarachnoid hemorrhage (SAH). Erythrocyte lysate (hemolysate), oxyhemoglobin (OxyHb), and bloody cerebrospinal fluid (CSF) are the causative agents for vasospasm. However, the signal transductio...

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Bibliographic Details
Published in:Life sciences (1973) 2000, Vol.67 (10), p.1233-1242
Main Authors: Patlolla, Anita, Zubkov, Alexander, Parent, Andrew, Zhang, John
Format: Article
Language:English
Subjects:
Animals
Basilar Artery - drug effects
Basilar Artery - physiology
Cerebral vasospasm
Dogs
Enzyme Activation
Enzyme Inhibitors - pharmacology
Erythrocytes - chemistry
Female
Genistein - pharmacology
Hemolysis
Humans
In Vitro Techniques
Male
Organ culture
Oxyhemoglobins - pharmacology
Polyenes - pharmacology
Polyunsaturated Alkamides
Precipitin Tests
Protein-Tyrosine Kinases - antagonists & inhibitors
Proto-Oncogene Proteins p21(ras) - antagonists & inhibitors
Proto-Oncogene Proteins p21(ras) - metabolism
Rabbit basilar artery
Rabbits
Ras
Signal transduction
Subarachnoid Hemorrhage - blood
Subarachnoid Hemorrhage - cerebrospinal fluid
Vasospasm, Intracranial - blood
Vasospasm, Intracranial - cerebrospinal fluid
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Summary:Cerebral vasospasm is the major factor of mortality and morbidity in the patients who have an aneurysmal subarachnoid hemorrhage (SAH). Erythrocyte lysate (hemolysate), oxyhemoglobin (OxyHb), and bloody cerebrospinal fluid (CSF) are the causative agents for vasospasm. However, the signal transduction pathways for the action of these spasmogens are not clear. In this study, we examined the possible effect of these spasmogens on the p21Ras protein, an important factor in the signal cascade, in rabbit basilar artery. Hemolysate enhanced p21Ras precipitation over a 7-day period. The initial increase of p21Ras precipitation occurred after the tissues were incubated for 2 days with hemolysate. The peak effect of hemolysate, which was markedly increased compared with control (P
ISSN:0024-3205
1879-0631
DOI:10.1016/S0024-3205(00)00710-4