Suppression of natural killer cell activity by splenocyte transplantation in a rat model of endometriosis

BACKGROUND: One immune characteristic of endometriosis is a decrease in natural killer (NK) cell activity. This study was performed to determine whether an abnormal immune reaction in an endometriosis animal model could be transferred to an animal of the same species. METHODS: An endometriosis model...

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Bibliographic Details
Published in:Human reproduction (Oxford) 2002-06, Vol.17 (6), p.1453-1458
Main Authors: Ota, Hirotaka, Rong, Huang, Igarashi, Shinichi, Tanaka, Toshinobu
Format: Article
Language:English
Subjects:
Adoptive Transfer
Animals
Biological and medical sciences
Cytotoxicity, Immunologic
Disease Models, Animal
Endometriosis
Endometriosis - immunology
Endometriosis - pathology
endometriosis model
Endometrium - immunology
Endometrium - pathology
Female
Female genital diseases
Gynecology. Andrology. Obstetrics
Humans
Immune Tolerance
In Vitro Techniques
Killer Cells, Natural - immunology
Macrophages - immunology
Medical sciences
natural killer cell activity
Non tumoral diseases
Rats
Rats, Wistar
Spleen - cytology
Spleen - immunology
Spleen - transplantation
splenocyte
transplantation
Transplantation, Homologous
Tumor Cells, Cultured
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Summary:BACKGROUND: One immune characteristic of endometriosis is a decrease in natural killer (NK) cell activity. This study was performed to determine whether an abnormal immune reaction in an endometriosis animal model could be transferred to an animal of the same species. METHODS: An endometriosis model was prepared using 8 week old female rats by grafting a small section of one uterine horn onto the mesentery, followed 4 weeks later by removal of the spleen and remaining uterine horn. Splenocytes, that had been depleted of macrophages were injected via the tail vein, and NK cell activity of splenocytes was determined 4 days later. The uterus was simultaneously investigated immunohistochemically for immune cells. There was a control group (untreated; group 1), a control–splenocyte injection group (group 2), an experimental endometriosis model group (group 3) and an endometriosis model splenocyte injection group (group 4). RESULTS: Splenocyte NK cell activity was decreased in group 3 to 42.0% of that of group 1 and in group 4 to 38.9%. Immunohistologically, the number of NK cells in groups 3 and 4 markedly decreased to 62.0 and 55.1% of group 1 respectively. CONCLUSION: It was demonstrated that abnormal immunity caused by allograft of immune cells could recur in an endometriosis rat model.
ISSN:0268-1161
1460-2350
DOI:10.1093/humrep/17.6.1453